Immobilizing hydroxycholesterol with apatite on titanium surfaces to induce ossification

Biomaterials Research
Cen ChenIn-Seop Lee

Abstract

Immobilizing bioactive molecules and osteoconductive apatite on titanium implants have investigated direct ossification. In this study, hydroxycholesterol (HC) was immobilized with apatite on titanium through simply adsorption or sandwich-like coating. Three kinds of hydroxycholesterol were chosen to induce ossification: 20α-hydroxycholesterol (20α- HC), 22(S)-hydroxycholesterol (22(S)-HC) and 25-hydroxycholesterol (25-HC).The effects of HC/apatite coating on ossification abilities were evaluated in vitro and in vivo. At 6 d, adsorbed apatite/25-HC and apatite/22(S)-HC coating exhibited some cytotoxicity, while the cell viability of apatite/20α-HC coating was similar as apatite coating. Immobilizing HC with apatite significantly enhanced the ALP activities compared with apatite coating. There was no significant difference in ALP value between adsorbed apatite/HC coating and sandwich-like apatite/HC/apatite coating. When compared with apatite coating, the mineral deposition improved by adsorbed HC with apatite at higher concentration in vivo. When compared with apatite coating, immobilizing HC with apatite coating induced the ossification in vitro and in vivo.

References

Apr 8, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Hoa Ton KhaFarhad Parhami
Apr 20, 2011·Journal of Cellular Biochemistry·Jared S JohnsonFarhad Parhami
Mar 30, 2012·Biomedical Materials·Esha S DesaiRichard A Gemeinhart
May 1, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Soon Eon BaeDong Keun Han
Dec 26, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Huanan WangSander C G Leeuwenburgh
May 7, 2013·Biomaterials·Raphael GuillotCatherine Picart
Jun 4, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hong Jae LeeSang Cheon Lee
Jun 21, 2013·Biomedical Materials·Pinar Yilgor HuriVasif Hasirci
Apr 29, 2014·Biomedical Materials·Deborah ViolantRicardo Patricio Teles

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Methods Mentioned

BETA
protein assay
dissection

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