PMID: 7536023Apr 1, 1995Paper

Immortalisation of human oesophageal epithelial cells by a recombinant SV40 adenovirus vector

British Journal of Cancer
S InokuchiT Mitomi

Abstract

We introduced the origin-defective SV40 early gene into cultured human oesophageal epithelial cells by infection of a recombinant SV40 adenovirus vector. The virus-infected cells formed colonies 3-4 weeks after infection in medium containing fetal calf serum. When the cells derived from 'serum-resistant' colonies were then maintained in the serum-free medium with a low calcium ion concentration, some of them passed the cell crisis and kept growing for over 12 months. These cells, regarded as immortalised cells, resembled the primarily cultured oesophageal epithelial cells in morphology and had some of their original characteristics. Treatment of the cells with a high calcium concentration induced phenotypic changes. These cells still responded to transforming growth factor beta. When the immortalised cells were injected into severe combined immunodeficient mice, they transiently formed epithelial cysts, although the typical differentiation pattern of the oesophageal epithelium was not observed. These cysts regressed within 2 months without development into tumours. The results indicated that human oesophageal epithelial cells were reproducibly immortalised by infection with a recombinant SV40 adenovirus vector at relatively hig...Continue Reading

Citations

Jan 10, 2019·The Laryngoscope·Jason PowellChris Ward
Jan 12, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xia ChenSusan L Thibeault
Dec 21, 2006·Differentiation; Research in Biological Diversity·Mariana Roesch-ElyPascal Tomakidi

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