Immortalization and characterization of bone marrow stromal fibroblasts from a patient with a loss of function mutation in the estrogen receptor-alpha gene

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
S C DieudonnéM F Young

Abstract

A male patient with abnormal postpubertal bone elongation was shown earlier to have a mutation in both alleles of the estrogen receptor, resulting in a nonfunctional gene. Marrow stromal fibroblasts (MSFs) derived from this patient were called HERKOs (human estrogen receptor knock outs), and in order to obtain continuous HERKO cell lines, they were immortalized using a recombinant adenovirus-origin-minus SV40 virus. MSFs are unique cells because they support hematopoesis and contain a mixed population of precursor cells for bone, cartilage, and fat. Three established cell lines (HERKO2, HERKO4, and HERKO7) were characterized and compared with the heterogeneous population of nonimmortalized HERKOs for their osteogenic potential. We performed Northern analysis of matrix genes implicated in bone development and metabolism and an in vivo bone formation assay by transplanting the cells subcutaneously into immunodeficient mice. All three HERKO lines expressed high amounts of collagen 1A1, osteopontin, osteonectin, fibronectin, decorin, biglycan, and alkaline phosphatase. Except for osteopontin, expression of these genes was slightly lower compared with nonimmortalized HERKOs. In the in vivo bone formation assay, the heterogeneous pop...Continue Reading

References

Jul 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K IbarakiM F Young
Sep 1, 1989·Calcified Tissue International·M MetsärantaE Vuorio
Sep 1, 1985·Calcified Tissue International·P G Robey, J D Termine
Jan 1, 1988·Journal of Cell Science. Supplement·M Owen
May 1, 1987·Cell and Tissue Kinetics·A J FriedensteinU V Gerasimov
Jan 1, 1985·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·H A NeumannG W Löhr
Feb 13, 1984·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M J Glimcher
Jun 1, 1994·Endocrine Reviews·R T TurnerT C Spelsberg
Jun 1, 1994·Endocrine Reviews·E R SimpsonM D Michael
Oct 20, 1994·The New England Journal of Medicine·E P SmithK S Korach
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D B LubahnO Smithies
Jan 1, 1993·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S C ManolagasT Bellido
Dec 1, 1995·The Journal of Clinical Endocrinology and Metabolism·A MorishimaK Qin
Nov 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N S FedarkoJ R Shapiro
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·G G KuiperJ A Gustafsson
Dec 1, 1995·Clinical Endocrinology·S G SouleH S Jacobs
Jul 10, 1997·The New England Journal of Medicine·C CaraniE R Simpson
Sep 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S A KuznetsovP G Robey
Sep 1, 1985·Calcified Tissue International·Pamela Gehron Robey, John D Termine

❮ Previous
Next ❯

Citations

May 29, 2008·The Journal of Clinical Endocrinology and Metabolism·Eric P SmithKenneth S Korach
May 18, 2016·International Journal of Molecular Sciences·Paola BendinelliMaria Alfonsina Desiderio
Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P H KrebsbachP G Robey
Jun 16, 1999·Endocrine Reviews·J F Couse, K S Korach
May 22, 2008·Journal of Surgical Oncology·Bhuvanesh Singh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Endocrinology and Metabolism
Kathleen E McDougallJonathan H Tobias
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Andrei S ChaginC Ohlsson
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Marie K LindbergC Ohlsson
Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
S H WindahlG Andersson
Trends in Endocrinology and Metabolism : TEM
Vincent Giguère
© 2022 Meta ULC. All rights reserved