Immortalization and characterization of human myometrial cells from term-pregnant patients using a telomerase expression vector

Molecular Human Reproduction
Melvyn S SoloffGarland D Anderson

Abstract

An examination of cellular processes involved in myometrial function has been greatly assisted by the use of human myometrial cells in primary culture. However, these cells can be used only for several passages before they senesce, and responses to various agents change with time in culture. The use of transformed cells is limited, as they can be polynucleated and can lose or gain chromosomes. We have developed three telomerase-immortalized cell lines from term-pregnant human myometrium to eliminate variability between passage numbers and allow genetic manipulations of myometrial cells to fully characterize signal pathways. These cells have a normal karyotype and were verified to be uterine smooth muscle by immunocytochemical staining for smooth muscle cell-specific alpha-actin and high affinity oxytocin antagonist binding sites. The three cell lines and the cells in primary culture from which they were derived were examined by cDNA microarray analysis. Of >10 000 expressed genes, there were consistent changes in the expression of approximately 1% in the three immortalized cell lines. We were unable to detect any significant differences between primary and immortalized cells in signal pathways such as epidermal growth factor-st...Continue Reading

Citations

Jul 29, 2011·Reproductive Sciences·Melvyn S SoloffSarah K England
Oct 6, 2005·Journal of the Society for Gynecologic Investigation·Barbara M SanbornLidiya Babich
Dec 2, 2006·Obstetrics and Gynecology·Melissa J WentzAyman Al-Hendy
Jan 15, 2020·American Journal of Physiology. Endocrinology and Metabolism·Chinwendu AmazuSarah K England
Dec 14, 2006·American Journal of Physiology. Endocrinology and Metabolism·Yow-Jiun JengMelvyn S Soloff
Mar 15, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Noriomi MatsumuraShingo Fujii
Feb 9, 2021·Experimental Biology and Medicine·Antonina P Maxey, Megan L McCain
Jun 11, 2005·Science·Cheryl Lyn Walker, Elizabeth A Stewart

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