PMID: 9183749Jun 20, 1997Paper

Immortalization and controlled in vitro differentiation of murine multipotent neural crest stem cells

Journal of Neurobiology
M S Rao, D J Anderson

Abstract

To isolate mouse neural crest stem cells, we have generated a rat monoclonal antibody to murine neurotrophin receptor (p75). We have immortalized p75+ murine neural crest cells by expression of v-myc, and have isolated several clonal cell lines. These lines can be maintained in an undifferentiated state, or induced to differentiate by changing the culture conditions. One of these cell lines, MONC-1, is capable of generating peripheral neurons, glia, and melanocytic cells. Importantly, most individual MONC-1 cells are multipotent when analyzed at clonal density. The neurons that differentiate under standard conditions have an autonomic-like phenotype, but under different conditions can express markers of other peripheral neuronal lineages. These lines therefore exhibit a similar differentiation potential as their normal counterparts. Furthermore, they can be genetically modified or generated from mice of different genetic backgrounds, providing a useful tool for molecular studies of neural crest development.

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Oct 1, 1992·Current Opinion in Neurobiology·K R Jessen, R Mirsky
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P S JatD Kioussis
Aug 1, 1991·Trends in Neurosciences·F H GageL J Fisher
Jan 1, 1991·Glia·K R Jessen, R Mirsky
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M MurphyP F Bartlett
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A BaroffioN M Le Douarin
Sep 1, 1989·Journal of Neuroscience Research·O BernardP F Bartlett
Dec 1, 1989·Neuron·M Bronner-Fraser, S Fraser
Jan 1, 1989·Annual Review of Neuroscience·C L Cepko
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M PeacockeB A Gilchrest
Aug 1, 1988·Neuron·K FrederiksenR McKay
Nov 21, 1986·Cell·D JohnsonM Chao
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·P F BartlettO Bernard
Jun 1, 1988·Cell Structure and Function·K ItoT Takeuchi
Jun 1, 1993·Developmental Biology·K ItoM Sieber-Blum
Sep 1, 1993·Developmental Biology·K Morrison-Graham, J A Weston
Nov 18, 1994·Brain Research. Developmental Brain Research·S G Matsumoto
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·M J Fann, P H Patterson

❮ Previous
Next ❯

Citations

Oct 12, 1999·Biotechnology and Bioengineering·M S KallosA L Vescovi
Oct 11, 2003·Current Opinion in Genetics & Development·Nicole M Le Douarin, Elisabeth Dupin
Oct 1, 2011·Nature Protocols·Heather Etchevers
Mar 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Quenten SchwarzChristiana Ruhrberg
Aug 15, 2012·Stem Cells and Development·Mamoru IshiiRobert E Maxson
Oct 14, 2000·Annual Review of Cell and Developmental Biology·C GrandoriR N Eisenman
Jun 8, 2002·Annual Review of Neuroscience·David I Gottlieb
Jan 29, 2013·Cilia·Marc August WillaredtKerry L Tucker
Nov 19, 2010·PloS One·Carol Lynn CurchoeAlexey V Terskikh
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·A V TerskikhI L Weissman
Jun 22, 2000·Journal of Medical Ethics·J R Meyer
Jan 26, 2002·Molecular and Cellular Biology·Leiming LiAkira Imamoto
Feb 13, 2003·Nucleic Acids Research·Shiyou ChenRobert J Lechleider
May 1, 2009·Development·Julaine Roffers-Agarwal, Laura S Gammill
Dec 24, 2010·Journal of Visualized Experiments : JoVE·Kristian PajtlerJohannes Hubertus Schulte
Feb 24, 2012·Journal of Neurophysiology·J M HawrylukE Arrigoni
Jun 15, 2007·Journal of the Peripheral Nervous System : JPNS·Weiran ChenAhmet Höke
May 2, 2017·Oncogene·R R OlsenK W Freeman
Aug 11, 2004·Developmental Biology·Arhat Abzhanov, Clifford J Tabin
Jan 29, 2018·Cell and Tissue Research·Isabelle Janoueix-LeroseyHermann Rohrer
Mar 30, 2004·Genesis : the Journal of Genetics and Development·Richard JägerHubert Schorle
Oct 2, 2015·Journal of Cellular Physiology·Ning Shi, Shi-You Chen
Feb 5, 2011·Circulation Research·Mark W MajeskyVirginia J Hoglund
May 13, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Meena S Kumar, Gary K Owens
Jun 17, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Changqing XieY Eugene Chen
Sep 8, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mariana Delfino-MachínRobert N Kelsh
Sep 6, 2007·Journal of Cellular Physiology·Hong-Yun Li, Xin-Fu Zhou
Jul 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A J Kalyani, M S Rao
Apr 16, 1998·The Journal of Biological Chemistry·M K JainM E Lee

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.