Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Nobuyuki KamataShigeru Yasumoto

Abstract

Human telomerase reverse transcriptase (hTERT) is catalytic subunit of human telomerase. We studied the immortalization of a series of human dental and periodontal cells by ectopic expression of hTERT and co-expression of hTERT with human papilloma virus 16 (HPV16) or simian virus 40 (SV40). Differentiation abilities of the established cell lines were studied in terms of the mineralized matrix formation and gene expression. We established immortalized gingival fibroblasts by hTERT, dental papilla and periodontal ligament cells by hTERT and HPV16, and pulp cells by hTERT and SV40. The papilla and pulp cells showed mineralization and dentin sialophosphoprotein (DSPP) expression when cultured in the presence of beta-glycerophosphate. The immortalized periodontal ligament cells did not show mineralization or DSPP expression, although expressions of alkaline phosphatase, osteopontin and osteocalcin were detected. These cell lines will be useful tools for studying the repair and regeneration of dental and periodontal tissues and various diseases including odontogenic tumors.

Citations

Nov 11, 2011·Journal of Materials Science. Materials in Medicine·Rasika P IlleperumaJin Kim
Jan 10, 2013·In Vitro Cellular & Developmental Biology. Animal·Heng LinZhi Chen
Aug 26, 2011·Journal of Dental Research·K OhtaT Kawai
Mar 11, 2006·Okajimas Folia Anatomica Japonica·Shunji KumabeYasutomo Iwai
Apr 11, 2014·In Vitro Cellular & Developmental Biology. Animal·Ha Le Bao TranLan Thi Quynh Ngo
Jul 26, 2011·Neurochemical Research·Mehmet E YalvaçAndrás Palotás
Apr 14, 2015·Periodontology 2000·Matthias ChiquetDimitris Kletsas
Nov 14, 2012·Journal of Endodontics·Min-Ching WangKuo-Wei Chang
Feb 24, 2009·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Bong-Wook ParkJune-Ho Byun
Feb 11, 2009·Molecular Carcinogenesis·Masaaki TatsukaTakashi Takata
Jan 3, 2013·Microbiology and Immunology·Akiko FukuiNobuyuki Kamata
Jul 11, 2012·International Endodontic Journal·J YonehiroF Nishimura
Oct 24, 2006·Advanced Drug Delivery Reviews·Shivaram SelvamMelvin D Trousdale
Mar 7, 2006·Biochemical and Biophysical Research Communications·Etsuko IkedaHajime Ohgushi
Nov 22, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H BabichH L Zuckerbraun
Apr 8, 2011·Archives of Oral Biology·Julie Teresa MarchesanYvonne L Kapila
Feb 18, 2015·Journal of Dentistry·Yide HuangYao Lin
Oct 1, 2010·FEMS Immunology and Medical Microbiology·Kouji OhtaNobuyuki Kamata
Nov 26, 2008·Journal of Dental Research·K OhtaN Kamata
Dec 26, 2006·Journal of Dental Research·H AgataM Ueda
Jul 10, 2009·Journal of Dental Research·H ShigeishiN Kamata
Jan 30, 2010·Journal of Dental Research·P-S HungK-W Chang
Oct 20, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hiroko HatanoNobuyuki Kamata
Jan 19, 2007·Skin Pharmacology and Physiology·A WelkG Daeschlein
Feb 6, 2019·International Journal of Molecular Sciences·Takeo KarakidaYasuo Yamakoshi
Jan 23, 2020·International Journal of Molecular Sciences·Gundula Schulze-TanzilSilke Schwarz
Jun 15, 2013·Macromolecular Bioscience·Obi EgbuniweLucy Di Silvio
Aug 22, 2018·International Journal of Molecular Sciences·Shunjiro YamakawaNoriyasu Hosoya

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