PMID: 3745269Sep 1, 1986Paper

Immortalization of human lymphocytes by fusion with cytoplasts of transformed mouse L cells

The Journal of Cell Biology
H AbkenK Willecke

Abstract

Fusion of mouse L929 cytoplasts with human peripheral blood lymphocytes induced lymphocyte proliferation that gave rise to lymphoid cell lines of B and T cell origin with unlimited growth potential. The immortalized cell lines were routinely grown in standard medium supplemented with fetal calf serum. Furthermore these cell lines could be propagated in chemically defined serum-free media. Each establishment of lymphoid cell lines was preceded by a proliferation phase 2 wk after cytoplast/cell fusion, which appears to be a necessary step in the immortalization process. The immortalized cells have a nearly normal human karyotype, do not form colonies in soft agar medium, and are not tumorigenic in nude mice. Cloned B cell lines produced human immunoglobulins of heavy and light chain types. No cross-reaction with DNA of herpes simplex virus, human cytomegalovirus, human T cell leukemia/lymphoma virus I and II, or polyoma virus was detected in the genome of immortalized cell lines by Southern blot hybridization. Furthermore B and T cell lines were established that appear to be free of Epstein-Barr virus genome.

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M BreindlR Jaenisch
Apr 7, 1975·Biochemical and Biophysical Research Communications·M H Wigler, I B Weinstein
May 1, 1973·International Journal of Cancer. Journal International Du Cancer·B M Reedman, G Klein
Dec 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·L F KleinmanP Polgar
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D A GallowayJ K McDougall
Oct 15, 1981·Experientia·C GélinasM Bastin
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D KozborJ C Roder
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M SeikiM Yoshida
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A HendersonE Kieff
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·I S ChenD W Golde
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E P GelmannR C Gallo
Nov 18, 1983·Science·H LandR A Weinberg
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R J CoteL J Old
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·A L MaizelC G Sahasrabuddhe
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M PopovicR C Gallo
May 1, 1983·Biulleten' eksperimental'noĭ biologii i meditsiny·E Iu AllikmetsIu A Rovenskiĭ
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L V JohnsonL B Chen
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A MaizelR Ford
Jan 1, 1982·Immunological Reviews·P MarrackJ W Kappler
Nov 1, 1982·The Journal of Experimental Medicine·R L RaisonA Basten

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Citations

May 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·B A Israel, W I Schaeffer
Sep 1, 1987·Mutation Research·J W Shay, H Werbin
Jan 1, 1990·Free Radical Biology & Medicine·B Bandy, A J Davison
Feb 4, 2006·Reproductive Biomedicine Online·Nick StrelchenkoYury Verlinsky
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H AbkenK Willecke
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·H AbkenK Willecke
Sep 19, 2009·Traffic·Aurelie Lachish-ZalaitMichael Elbaum
Apr 1, 1992·Experimental Cell Research·Q C RyanD M Murasko

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