PMID: 9437508Jan 23, 1998Paper

Immortalization of human marrow stromal cells by retroviral transduction with a temperature sensitive oncogene: identification of bipotential precursor cells capable of directed differentiation to either an osteoblast or adipocyte phenotype

Bone
A HoughtonB M Stringer

Abstract

The etiology of osteoporosis is multifactorial, but there is evidence from both animal and human studies that the volume of marrow adipose tissue increases when bone volume is reduced in osteoporosis. The cell-related mechanism that may account for this inverse relationship between the volume of marrow adipose tissue and bone remains to be clarified, although it is known that both adipocytes and osteoblasts are derived from stromal cells precursors in bone marrow. We report that retroviral transduction with a temperature-sensitive oncogene (SV40 large T antigen) can generate bipotential cell lines from human marrow stroma that are capable of directed differentiation, in vitro, down either an osteogenic or adipocytic lineage pathway. One such clone, designated hOP 7, expresses type alpha 1(I) procollagen and has low alkaline phosphatase (AP) activity under basal culture conditions that is reminiscent of an osteoprogenitor cell. Exposure of hOP 7 cells to dexamethasone upregulates AP activity and enables the cells to mineralize their extracellular matrix. Also, treatment with calcitriol induces osteocalcin expression and both PTH and PGE2 induce/augment cAMP formation. Incubation with normal rabbit serum, however, causes the cell...Continue Reading

References

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Citations

Mar 23, 2011·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Toshihisa TsurutaYoshikazu Okada
Apr 20, 2007·International Journal of Clinical Oncology·Hajime HosoiTohru Sugimoto
Jan 25, 2003·Tissue Engineering·Karine AnselmePierre Hardouin
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Nov 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C G Bellows, J N Heersche
Sep 8, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·O FromiguéJ J Body
Aug 11, 2007·Journal of Occupational Health·Cui-Qin XiangSheng-Nian Zhang
May 1, 2007·Regenerative Medicine·Pierre J Marie, Olivia Fromigué
Jun 30, 2015·Progress in Lipid Research·Alexandrine DuringPierre Hardouin
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Dec 5, 2019·Calcified Tissue International·Alexandrine DuringCécile Olejnik
Aug 21, 2020·Biochimie·Alexandrine During

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