Immortalization of human nucleus pulposus cells by a recombinant SV40 adenovirus vector: establishment of a novel cell line for the study of human nucleus pulposus cells

Spine
Daisuke SakaiTomomitsu Hotta

Abstract

Establishment and characterization of a de novo cell line derived from human nucleus pulposus cells using a recombinant simian virus 40 (SV40) adenovirus vector. To assess the feasibility of human nucleus pulposus cell line procurement and to evaluate the character of the resultant outcome to better understand the nature of human nucleus pulposus cells. Despite recent advances in disc cell biologic research, the fundamental nature of nucleus pulposus cells, especially in the context of human cell lines, is still not well understood. Therefore, a broad-based analysis of these cells is of significant necessity. Because of the limited amount of existing human cells, establishment of an immortal cell line would greatly facilitate resource supply. After release of informed consent, tissue samples of nucleus pulposus were obtained from the lumbar intervertebral disc of a 19-year-old man undergoing anterior fusion for burst fracture. Samples with no apparent damage were selected and digested enzymatically for primary culture and then were infected with recombinant SV40 adenovirus vector (Ad/SV40). The infected cells were maintained in culture for more than 40 population doublings, after which they were considered immortalized. Next, c...Continue Reading

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Citations

Dec 1, 2010·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Yun-Tao WangFeng Wang
Mar 16, 2005·Spine·James W Ogilvie
May 15, 2007·Spine·Sylvia A ChungAshish D Diwan
Jan 25, 2005·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Joji Mochida
Nov 30, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Junya YamamotoKotaro Nishida
Mar 16, 2016·BMC Musculoskeletal Disorders·Guus G H van den AkkerJan Willem Voncken
Jul 15, 2015·The Journal of Orthopaedic and Sports Physical Therapy·Edith Elgueta-CancinoPaul Hodges
Jun 2, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nobuyuki FujitaMorio Matsumoto

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