Immortalization of Primary Keratinocytes and Its Application to Skin Research

Biomolecules & Therapeutics
Moonju Choi, Choongho Lee

Abstract

As a major component of the epidermal tissue, a primary keratinocyte has served as an essential tool not only for the study of pathogenesis of skin-related diseases but also for the assessment of potential toxicities of various chemicals used in cosmetics. However, its short lifespan in ex vivo setting has been a great hurdle for many practical applications. Therefore, a number of immortalization attempts have been made with success to overcome this limitation. In order to understand the immortalization process of a primary keratinocyte, several key biological phenomena governing its lifespan will be reviewed first. Then, various immortalization methods for the establishment of stable keratinocyte cell lines will be explained. Finally, its application to a three-dimensional skin culture system will be described.

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Citations

Jul 30, 2020·International Journal of Molecular Sciences·Marta GerasymchukIgor Kovalchuk
Jul 3, 2020·Stem Cells International·María Piñeiro-RamilSilvia Díaz-Prado
Sep 9, 2018·Scientific Reports·Tanja WagnerMichael Mildner
Jun 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Kim E BoonekampHans Clevers
Dec 6, 2020·Burns : Journal of the International Society for Burn Injuries·Sina KardehShima Faezi-Marian
Jun 3, 2021·NPJ Microgravity·Dong Hyun ChoiSungwan Kim
Aug 11, 2021·Polymers·Alena Opálková ŠiškováAnita Eckstein Andicsová
Aug 27, 2021·Stem Cell Research & Therapy·Akiko Tanuma-TakahashiAkihiro Umezawa

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Methods Mentioned

BETA
nucleotide exchange

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