PMID: 701817Jan 1, 1978Paper

Immune adherence: a quantitative and kinetic analysis

Journal of Immunological Methods
T Sekine

Abstract

Quantitative and kinetic analysis of the immune-adherence reaction (IA) between C3b fragments and IA receptors as an agglutination reaction is difficult. Analysis is possible, however, by use of radio-iodinated bovine serum albumin as antigen at low concentrations (less than 200 ng/ml) and optimal concentration of antibody to avoid precipitation of antigen--antibody complexes with human erythrocytes without participation of complement. Antigen and antibody are reacted at 37 degree C, complement is added, the mixture incubated and human erythrocytes added; after further incubation, ice-cold EDTA containing buffer is added and the erythrocytes centrifuged and assayed for radioactivity. Control cells reacted with heated guinea pig serum retained less than 5% of the added radioactivity. The method facilitates measurement of IA reactivity and permits more detailed analysis of the mechanism underlying the reaction.

References

May 29, 1975·The New England Journal of Medicine·S KrugmanC Lattimer
May 1, 1976·The Journal of Experimental Medicine·D W Mason
Nov 1, 1968·The Journal of Experimental Medicine·W H Lay, V Nussenzweig
Mar 15, 1969·International Journal of Cancer. Journal International Du Cancer·K NishiokaS Takeuchi

❮ Previous
Next ❯

Citations

Jul 1, 1981·Journal of Neurosurgery·J A GarsonP L Kornblith

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.