Immune cell infiltration and cytokine secretion analysis reveal a non-inflammatory microenvironment of medulloblastoma

Oncology Letters
Shuo DiaoChunjiang Yu

Abstract

Medulloblastoma (MB) is the most common lethal malignant pediatric brain tumor. Adjuvant immunotherapy for medulloblastoma has been proposed in both pre-clinical and clinical practice. To provide a precision strategy of designing immunotherapy for MB, the present study performed a descriptive analysis of immune microenvironment in a cohort and compared the differences between four subgroups of MB. Subtypes (WNT, SHH Group 3 and Group 4) of medulloblastoma were identified using K-means clustering according to the expression of signature genes. Tumor infiltrating immune cell population was assessed by both bio-informative algorithm based on gene expression and immunohistochemistry staining. Cytokines in tumor microenvironment were detected using Luminex. Gene Set Enrichment Analysis demonstrated a raised immune response in the SHH subgroup. Lymphocyte infiltration was low in all four subgroups, while more CD4+ T cells were observed in the Group 4 subtype. Programmed cell death protein 1 (PD1)/ ligand 1 (PD-L1) expression was absent in the cohort. The SHH subtype recruited more activated tumor associated macrophage/microglia compared with the other subgroups. Cytokines within the MB microenvironment were low compared with the glio...Continue Reading

Citations

Feb 9, 2021·International Journal of Radiation Oncology, Biology, Physics·Clemens GrassbergerTorunn I Yock
Jul 31, 2021·Current Treatment Options in Oncology·Zahraa F AudiSanaa M Nabha
Sep 5, 2021·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Lourdes Alvarez-ArellanoCarmen Maldonado-Bernal

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Methods Mentioned

BETA
surgical resection
cDNA library
PCR
Seq
Assay
RNA-Seq

Software Mentioned

- counter
GSEA
SPSS
MCP
GraphPad Prism
ImageJ
GraphPad
GESA
Agilent2200

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