PMID: 2501510Jul 1, 1989Paper

Immune enhancement by tumor necrosis factor-alpha improves antibiotic efficacy after hemorrhagic shock

The Journal of Trauma
D H LivingstonG Sonnenfeld

Abstract

Immunomodulation with cytokines produced by recombinant DNA technology may be useful in combatting the increased susceptibility to infection seen after shock and trauma. We investigated the effect of tumor necrosis factor (TNF) alone and in combination with an antibiotic in a model of infection after shock. Interactions of TNF with another cytokine, interferon-gamma (IFN-gamma), were also examined. Sprague-Dawley rats were bled to maintain blood pressure at 45 mm Hg for 45 minutes and then resuscitated with shed blood and saline. Animals were inoculated with 10(8) S. aureus subcutaneously and placed into one of seven treatment groups: 1) control; 2) CEF-cefazolin, 30 mg/kg IP, 30 minutes before and 4 hours after inoculation; 3) IFN-recombinant rat IFN-gamma, 7,500 U SQ, 30 minutes after inoculation and daily for 3 days; 4) TNF-recombinant human TNF, 7,500 U SQ, 30 minutes after inoculation and daily for 3 days; 5) CEF + IFN as in 2 and 3; 6) CEF + TNF as in 2 and 4; 7) CEF + IFN + TNF as in 2, 3, and 4. Animals were sacrificed on day 7 and abscess number, diameter, and weight were measured. CEF reduced abscess diameter and weight following hemorrhagic shock, but did not affect abscess number. IFN-gamma and TNF alone did not red...Continue Reading

Citations

Jan 1, 1996·Biotherapy·M Degré
Jun 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M Degré
Feb 1, 1993·American Journal of Surgery·D H Livingston
Jan 24, 2008·Critical Care : the Official Journal of the Critical Care Forum·Arwed HostmannSven K Tschoeke
Jul 1, 1990·The Australian and New Zealand Journal of Surgery·J L McCall, B R Parry
Aug 1, 1992·Anaesthesia and Intensive Care·R Bellomo
Nov 1, 1991·The Journal of Surgical Research·C S Rock, S F Lowry

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