Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against Corynebacterium pseudotuberculosis

Bioinformatics and Biology Insights
Daniela Droppa-AlmeidaFrancine F Padilha

Abstract

Caseous lymphadenitis (CLA) is a disease caused by Corynebacterium pseudotuberculosis bacteria that affects sheep and goats. The absence of a serologic diagnose is a factor that contributes for the disease dissemination, and due to the formation of granuloma, the treatment is very expensive. Therefore, prophylaxis is the approach with best cost-benefit relation; however, it still lacks an effective vaccine. In this sense, this work seeks to apply bioinformatic tools to design an effective vaccine against CLA, using CP40 protein as standard for the design of immunodominant epitopes, from which a total of 6 sequences were obtained, varying from 10 to 16 amino acid residues. The evaluation of different properties of the vaccines showed that the vaccine is a potent and nonallergenic antigen remaining stable in a wide range of temperatures. The initial tertiary structure of the vaccine was then predicted and a model selected. Later, the process of CP40 protein and TLR2 receptor binding was performed, presenting interaction with this receptor, which plays an important role in the activation of the immune response.

References

Aug 11, 1998·Zentralblatt für Veterinärmedizin. Reihe A·F AntonF Gaytán
Aug 23, 2001·The Veterinary Clinics of North America. Food Animal Practice·L H Williamson
Jan 31, 2003·Proteins·Simon C LovellDavid C Richardson
Jul 18, 2006·Nucleic Acids Research·Sudipto Saha, G P S Raghava
Jan 9, 2007·BMC Bioinformatics·Irini A Doytchinova, Darren R Flower
May 23, 2007·Nucleic Acids Research·Markus Wiederstein, Manfred J Sippl
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
Jun 6, 2013·Nucleic Acids Research·Lim HeoChaok Seok

❮ Previous
Next ❯

Citations

Jul 10, 2019·Microbial Biotechnology·Thiago Doria BarralRicardo Dias Portela
Apr 10, 2019·Clinical and Experimental Immunology·J WallisR C Carlisle
Mar 7, 2021·Microorganisms·Doglas PariseJan Baumbach
Sep 25, 2021·Critical Reviews in Microbiology·Roghayeh MohammadzadehHadi Farsiani
Oct 7, 2021·Scientific Reports·Adewale Oluwaseun FadakaMervin Meyer

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear
x-ray crystallography
x-ray
nuclear magnetic resonance
NMR

Software Mentioned

3Dpro
ANTIGENpro
SignalP
ProtParam
Galaxy TBM
PSORTb
HA
BepiPred
VaxiJen
MODELLERCSA

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.