Immune monitoring of patients with septic shock by measurement of intraleukocyte cytokines

Intensive Care Medicine
Thierry FumeauxJérôme Pugin

Abstract

To assess the immune competence of patients presenting with septic shock by measuring on-line the production of intracellular cytokines by circulating leukocytes. Prospective study in a 18-bed medical intensive care unit of a university hospital. 21 patients with septic shock, and 11 volunteers. Single-step isolation of leukocytes from whole blood obtained within the first 24 h after admission. Leukocytes were fixed immediately or after treatment with lipopolysaccharide (LPS) and/or heterologous plasma. Leukocytes were permeabilized, and the intracellular cytokine expression of TNF-alpha and IL-10 was quantified by immunostaining and flow cytometry. LPS treatment significantly increased monocyte intracellular cytokine TNF-alpha and IL-10 as well as lymphocyte intracellular cytokine IL-10 in normal leukocytes. Septic monocytes and granulocytes had nonstimulated intracellular cytokine TNF-alpha concentrations lower than those measured in volunteers and were severely hyporesponsive to LPS. These phenotypic changes were correlated with disease severity and could be reproduced by treatment of normal leukocytes with plasma from patients with septic shock. Intracellular cytokine staining is a simple and rapid method to assess in situ ...Continue Reading

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Citations

Jul 17, 2009·Critical Care : the Official Journal of the Critical Care Forum·Holger PoehlmannChristian Meisel
Apr 14, 2010·Critical Care : the Official Journal of the Critical Care Forum·Oh Yoen KimMinou Adib-Conquy
Feb 18, 2009·Intensive Care Medicine·Masja LeendertseTom van der Poll
Jun 23, 2009·The Journal of Surgical Research·Teru YabukiToshio Nakatani
Jan 29, 2008·Journal of the American College of Surgeons·Steven J SchwulstRichard S Hotchkiss
Jul 2, 2015·Cytometry. Part B, Clinical Cytometry·Guillaume Monneret, Fabienne Venet
Aug 6, 2013·Human Immunology·Eleonora BaffariAlberto Bergamini
Nov 3, 2017·Journal of Biomedical Materials Research. Part a·Hagen BombergSven O Schneider

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