Immune Profiling Panel: A Proof-of-Concept Study of a New Multiplex Molecular Tool to Assess the Immune Status of Critically Ill Patients.

The Journal of Infectious Diseases
Dina M TawfikJulien Textoris

Abstract

Critical illness such as sepsis is a life-threatening syndrome defined as a dysregulated host response to infection and is characterized by patients exhibiting impaired immune response. In the field of diagnosis, a gap still remains in identifying the immune profile of critically ill patients in the intensive care unit (ICU). A new multiplex immune profiling panel (IPP) prototype was assessed for its ability to semiquantify messenger RNA immune-related markers directly from blood, using the FilmArray System, in less than an hour. Samples from 30 healthy volunteers were used for the technical assessment of the IPP tool. Then the tool was clinically assessed using samples from 10 healthy volunteers and 20 septic shock patients stratified using human leukocyte antigen-DR expression on monocytes (mHLA-DR). The IPP prototype consists of 16 biomarkers that target the immune response. The majority of the assays had a linear expression with different RNA inputs and a coefficient of determination (R2) > 0.8. Results from the IPP pouch were comparable to standard quantitative polymerase chain reaction and the assays were within the limits of agreement in Bland-Altman analysis. Quantification cycle values of the target genes were normaliz...Continue Reading

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Citations

Jul 22, 2020·The Journal of Infectious Diseases·Edward J Septimus
Aug 7, 2021·The Journal of Experimental Medicine·Jonathan LopezSophie Trouillet-Assant

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Methods Mentioned

BETA
PCR
flow cytometry
reverse-transcription PCR
unique flow

Clinical Trials Mentioned

NCT02803346

Software Mentioned

IPP
FilmArray
LightCycler
R

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