Immune Regulation of RAW264.7 Cells In Vitro by Flavonoids from Astragalus complanatus via Activating the NF-κ B Signalling Pathway

Journal of Immunology Research
Yu LiJinjie Wu

Abstract

The current study aimed at investigating the effects of flavonoids from Astragalus complanatus (FAC) on the proliferation, the contents, and gene expression levels of cytokines, secretion of surface stimulating factors, cell cycle, and the expression level of the NF-κB signalling pathway in RAW264.7 cells. Our results revealed that compared with control group, the contents of IL-6, IL-1β, TNF-α, and NO and the mRNA expression levels of IL-6, IL-1β, TNF-α, and iNOS in FAC-treated groups significantly increased (p < 0.01). Moreover, FAC induced macrophage activation to release the above-mentioned mediators partly involved in NF-κB/MAPK signalling pathways. Therefore, FAC regulates immune function in RAW264.7 cells via activating the NF-κB signalling pathway. FAC could be applicable for agriculture, drug research, and food industry as a potent immune-modulatory agent.

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Citations

Dec 17, 2020·Phytotherapy Research : PTR·Bahare SalehiCarmen Sadaka
May 1, 2019·European Journal of Pharmacology·Sheng ZhaoGuangyi Jin
Sep 26, 2020·Current Topics in Medicinal Chemistry·Chen XuBan-Feng Ruan
Oct 5, 2021·Experimental and Therapeutic Medicine·Bussarin TongmeeDoungporn Amornlerdpisan

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Methods Mentioned

BETA
ELISA
PCR
flow cytometry

Software Mentioned

SPSS

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