Immune response in the conjunctival epithelium of patients with dry eye

Experimental Eye Research
Stefano BarabinoMaurizio Rolando

Abstract

The aim of our project was to test the hypothesis that patients with dry eye have a significant degree of inflammation and lymphocyte infiltration in conjunctival epithelium by using flow cytometry analysis of cells stored in cell culture medium. Impression cytology specimens were collected in 15 normal subjects and 15 dry eye patients. Samples collected from the right eye were placed in Phosphate Buffered Saline containing 0.05% paraformaldehyde (PFA), and samples from the left eye in cell culture medium containing 10% foetal calf serum (FCS). Phenotypic analysis was performed on cells derived from 21 dry eye patients and 16 healthy controls. The cells collected in FCS were stained for the expression of CK19, CD3, CD4, CD8, CD56, CD19, CD20, CD14 and HLA-DR, and analyzed by flow cytometry. FCS samples contained a statistically increased number of cells when compared to PFA samples. No statistically significant differences were present in the number of CD45+CK19- cells, CD3+ and CD4+T cells, B and NK cells in dry eye patients compared to healthy controls. In the dry eye group there was a significant difference in the CD4/CD8 ratio respect to what observed in normal subjects, and an increased number of CD14+ cells. HLA-DR expres...Continue Reading

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Citations

Jan 11, 2012·Archives of Ophthalmology·William StevensonReza Dana
Jul 21, 2012·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Christina JacobiClaus Cursiefen
Jun 11, 2014·Nature Reviews. Rheumatology·Louis TongSalvatore Albani
Jan 31, 2013·International Reviews of Immunology·Michael E SternStephen C Pflugfelder
Jun 26, 2012·Survey of Ophthalmology·Samantha McGinnigleFrank Eperjesi
Mar 20, 2012·Progress in Retinal and Eye Research·Stefano BarabinoReza Dana
Mar 31, 2015·Experimental Eye Research·Rachel L RedfernAlison M McDermott
Oct 4, 2014·Saudi Journal of Ophthalmology : Official Journal of the Saudi Ophthalmological Society·Hui Lin, Samuel C Yiu
Apr 9, 2017·Acta Ophthalmologica·Christophe BaudouinUNKNOWN ODISSEY European Consensus Group Members
Apr 5, 2017·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Maurizio Rolando, Aldo Vagge
Dec 10, 2017·Scientific Reports·Yong-Soo ByunSo-Hyang Chung
Jan 11, 2019·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Kalaivarny GanesalingamJennifer P Craig
Jan 24, 2019·Pharmaceutical Research·Mitalee TamhaneVeena Viswanath
Dec 28, 2019·Pharmaceutics·Natallia DubashynskayaYury A Skorik
Apr 21, 2018·International Journal of Molecular Sciences·Pierre NicolleAnnabelle Reaux-Le Goazigo
Sep 19, 2018·International Journal of Molecular Sciences·Stephen C PflugfelderCintia S De Paiva
Dec 12, 2012·European Journal of Ophthalmology·Pasquale AragonaDomenico Puzzolo
Sep 7, 2019·Translational Vision Science & Technology·Neeta S RoyUNKNOWN for the Dry Eye Assessment and Management (DREAM) Study Group
Aug 31, 2018·Mediators of Inflammation·José L ReyesAlicia Vázquez Mendoza
Oct 2, 2019·Taiwan Journal of Ophthalmology·Zhang-Zhe Thia, Louis Tong
Feb 6, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Bo Ram KimJin Sook Yoon
Dec 10, 2020·International Journal of Molecular Sciences·Kazuo TsubotaReza Dana
Feb 24, 2021·The Ocular Surface·Jeremias G Galletti, Cintia S de Paiva
Apr 17, 2021·The Ocular Surface·Archana Padmanabhan NairSwaminathan Sethu
Jul 21, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Noor Haziq SalimanPhilip B Morgan
Sep 30, 2021·European Journal of Ophthalmology·Stefano BarabinoMaurizio Rolando

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