Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51

PloS One
Elaine Maria Seles DornelesAndrey Pereira Lage

Abstract

Brucella abortus S19 and RB51 strains have been successfully used to control bovine brucellosis worldwide; however, currently, most of our understanding of the protective immune response induced by vaccination comes from studies in mice. The aim of this study was to characterize and compare the immune responses induced in cattle prime-immunized with B. abortus S19 or RB51 and revaccinated with RB51. Female calves, aged 4 to 8 months, were vaccinated with either vaccine S19 (0.6-1.2 x 1011 CFU) or RB51 (1.3 x 1010 CFU) on day 0, and revaccinated with RB51 (1.3 x 1010 CFU) on day 365 of the experiment. Characterization of the immune response was performed using serum and peripheral blood mononuclear cells. Blood samples were collected on days 0, 28, 210, 365, 393 and 575 post-immunization. Results showed that S19 and RB51 vaccination induced an immune response characterized by proliferation of CD4+ and CD8+ T-cells; IFN-ɣ and IL-17A production by CD4+ T-cells; cytotoxic CD8+ T-cells; IL-6 secretion; CD4+ and CD8+ memory cells; antibodies of IgG1 class; and expression of the phenotypes of activation in T-cells. However, the immune response stimulated by S19 compared to RB51 showed higher persistency of IFN-ɣ and CD4+ memory cells,...Continue Reading

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Citations

Aug 19, 2016·PLoS Neglected Tropical Diseases·Aseem PandeyPaul de Figueiredo
Sep 20, 2020·Veterinary Medicine and Science·Shariful IslamSharmin Chowdhury
Oct 3, 2018·Tropical Medicine and Infectious Disease·Muhammad Zahoor Khan, Muhammad Zahoor
Jun 12, 2018·Journal of Immunology Research·Rongjiong ZhengKai Wang
May 22, 2018·Journal of the South African Veterinary Association·Gregory J G SimpsonJacques Godfroid
Sep 24, 2020·Microbiology and Immunology·Luciana Fachini CostaRenato Lima Santos
Feb 12, 2021·Frontiers in Immunology·Jamal Hussen, Hans-Joachim Schuberth
Sep 19, 2019·Vaccine: X·Slim ZribaAngela M Arenas-Gamboa
Oct 9, 2021·Tropical Animal Health and Production·M MelladoJ E García

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Methods Mentioned

BETA
light microscopy
protein
flow cytometry
ELISA
enzyme-linked immunosorbent assays
I-ELISA
flow

Software Mentioned

GraphPad
R
FlowJo
Epicalc
Graphpad PRISM
Stats
Primer Express

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