Immune response of the guinea pig to bovine parathyroid hormone antigen: influence of the booster dose on antisera titer and sensitivity

Journal of Immunoassay
P D'Amour, G Richer

Abstract

Thirty guinea pigs were immunized with 40 microgram of bovine parathyroid hormone, bPTH(1-84), and divided into 6 groups with a similar primary response. Each group was boosted twice with a different dose of antigen (0.04 to 40 microgram). The titer (30% binding of tracer) and affinity (% displacement with 320 pg of bPTH(1-84) were studied. The primary response was maximal at 61 days; titers were 1 x 10(-4) or less and affinity was low. The response was maximal 25 days after each booster. After the first, the highest titers, 5.2 +/- 2.6 x 10(-4) (mean +/- S.D.) were seen in the 40 microgram group and a positive correlation was observed between the dose of antigen and the titer (r = .7386, p less than 0.001) for doses greater than 0.6 microgram. This remained true after the second booster although titers were lower in all groups. Affinity was greater after the first booster (35.85 +/- 20.38%, n = 30) than after the second (23.24 +/- 20.73, n = 30, p less than 0.0025), but was similar in all groups. Selected antisera reacted with antigenic determinants in regions 1-34, 53-84 and 35-53 of the bPTH molecule. Cross-reactivity with human PTH(1-84) was maximal in the 53-84 region. In conclusion, antibodies against all regions of bPTH ...Continue Reading

References

Apr 9, 1974·Biochemistry·H T KeutmannJ L O'Riordan
Mar 1, 1971·The Journal of Endocrinology·G M AddisonJ L O'Riordan
Dec 1, 1971·The Journal of Clinical Endocrinology and Metabolism·J VaitukaitisG T Ross
Jan 1, 1971·The Journal of Clinical Investigation·C D ArnaudT Littledike
Jul 6, 1971·Biochemistry·H T KeutmannJ T Potts
Jun 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·E Reis, J M Canterbury
Apr 1, 1980·The Journal of Endocrinology·R M ManningJ L O'Riordan
May 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·S A BersonJ T Potts

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Citations

Jun 1, 1983·Journal of Clinical Pathology·J M Hows, J M Smith
Jun 1, 1983·Journal of Clinical Pathology·K S Zinneman, J D Chapman
Mar 1, 1984·The American Journal of Physiology·P D'Amour, P M Huet

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