Immune responses and protection obtained with rotavirus VP6 DNA vaccines given by intramuscular injection

Vaccine
K YangJ E Herrmann

Abstract

Intramuscular (i.m.) injection of murine VP6 DNA vaccines raised high titers of rotavirus-specific serum IgG and IgA antibodies in BALB/c mice. A Th1-like antibody response was generated based on the ratio of serum IgG2a to IgG1 antibodies. Rotavirus-specific serum IgA but not fecal IgA was detected in mice prior to rotavirus challenge. Partial protection against rotavirus challenge was achieved as measured by reduction of rotavirus antigen shedding in feces. A similar level of protection was found with a bovine rotavirus VP6 DNA vaccine against a murine rotavirus challenge, suggesting that heterologous protection can be obtained by immunizing with VP6 DNA vaccines. We did not directly test for cytotoxic T lymphocyte (CTL) activity, but in vivo depletion of CD8+ T cells in mice immunized with a murine VP6 DNA vaccine did not significantly change the duration of virus shedding or the pattern of protection obtained. This finding suggested that CD8+ CTL activity was not essential for the partial protection we obtained by i.m. immunization of mice with VP6 DNA vaccines.

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Citations

Nov 30, 2005·Transgenic Research·Eric SolerLouis-Marie Houdebine
Jun 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Andrea S LaimbacherCornel Fraefel
Sep 3, 2010·Clinical and Vaccine Immunology : CVI·Sangun LeeJohn E Herrmann
May 31, 2013·The Onderstepoort Journal of Veterinary Research·Tingting CuiSanjie Cao
Apr 25, 2012·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Shou-Chien ChenKow-Tong Chen
Jan 12, 2016·Human Vaccines & Immunotherapeutics·Fei SuWangxue Chen
Oct 21, 2004·Seminars in Pediatric Infectious Diseases·James P Nataro
Oct 12, 2015·World Journal of Pediatrics : WJP·Ching-Min WangKow-Tong Chen
Aug 13, 2005·Vaccine·John E Herrmann
Apr 8, 2015·Vaccine·O Badillo-GodinezF Esquivel-Guadarrama
May 30, 2015·Vaccine·Somayeh JalilvandZabihollah Shoja
Jan 8, 2019·Reviews in Medical Virology·Atefeh AfchangiZabihollah Shoja
Aug 1, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katharine M IrvineMatthew J Sweet
Nov 20, 2004·Immunology and Cell Biology·Manija AsifJohn W Lowenthal
Mar 11, 2005·Journal of Leukocyte Biology·Diego AlignaniMaría C Pistoresi-Palencia
Apr 19, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mila Ayash-RashkovskyZvi Bentwich

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