Immune status assay (ISA): a noninvasive procedure for studying allograft rejection

Transplant Immunology
Luis A FernandezMajed M Hamawy

Abstract

There is a need for a simple, sensitive, noninvasive technique for monitoring graft function. We report here on a simple assay called immune status assay (ISA) that determines the status of the graft by simply examining the activation status of blood T cells. Graft-derived fibroblasts were used as a source of alloantigens and the recipient blood as a source of allograft-specific peripheral blood lymphocytes (PBL). PBL were added to wells containing donor or third-party graft-derived fibroblasts in the presence or absence of interleukin-2 (IL-2). On day 4 [(3)H]thymidine incorporation was quantified after the cells were incubated for 3 days at 37 degrees C, in a 5% CO(2) water-jacketed incubator. The results were analyzed using the following equation: %IL2 - /IL2+ = ((mean[(3)H]thymidine uptake in the absence of IL - 2) / (mean [(3)H]thymidine uptake in the presence of IL - 2)) x 100. The ISA score (%IL-2 - /IL-2+) correlated strongly with the outcome of the graft, as it had a sensitivity of 82% for detecting rejections (14/17), and a specificity of 81% (30/37) for detecting non-rejections. Notably, the ISA detected immune T cell activation in the blood of graft rejecting subjects, which were not detected by currently used techn...Continue Reading

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Citations

Jul 16, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Josh Levitsky
Nov 3, 2009·Journal of Zhejiang University. Science. B·Yue WangZhi-qi Wang
Dec 2, 2011·Journal of the American Veterinary Medical Association·Boel A Fransson, Claude A Ragle
Dec 12, 2012·Critical Reviews in Food Science and Nutrition·Imran PashaMuhammad Umair Arshad
Apr 15, 2011·Critical Reviews in Food Science and Nutrition·Farhan SaeedMuhammad Tauseef Sultan
Sep 21, 2012·Expert Review of Clinical Immunology·Ali Zarrinpar, Ronald W Busuttil

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