IMMUNEPOTENT CRP induces cell cycle arrest and caspase-independent regulated cell death in HeLa cells through reactive oxygen species production

BMC Cancer
Ana Carolina Martínez-TorresCristina Rodríguez-Padilla

Abstract

Regulated cell death (RCD) is a mechanism by which the cell activates its own machinery to self-destruct. RCD is important for the maintenance of tissue homeostasis and its deregulation is involved in diseases such as cervical cancer. IMMUNEPOTENT CRP (I-CRP) is a dialyzable bovine leukocyte extract that contains transfer factors and acts as an immunomodulator, and can be cytotoxic to cancer cell lines and reduce tumor burden in vivo. Although I-CRP has shown to improve or modulate immune response in inflammation, infectious diseases and cancer, its widespread use has been limited by the absence of conclusive data on the molecular mechanism of its action. In this study we analyzed the mechanism by which I-CRP induces cytotoxicity in HeLa cells. We assessed cell viability, cell death, cell cycle, nuclear morphology and DNA integrity, caspase dependence and activity, mitochondrial membrane potential, and reactive oxygen species production. I-CRP diminishes cell viability in HeLa cells through a RCD pathway and induces cell cycle arrest in the G2/M phase. We show that the I-CRP induces caspase activation but cell death induction is independent of caspases, as observed by the use of a pan-caspase inhibitor, which blocked caspase ac...Continue Reading

References

May 1, 1995·The Journal of Experimental Medicine·H NakajimaP A Henkart
Dec 16, 1994·Science·L H Hartwell, M B Kastan
Sep 1, 1997·Nature Medicine·T WaldmanJ Williams
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·M Leist, M Jäättelä
Mar 5, 2003·Cellular and Molecular Life Sciences : CMLS·M ZafarullahM Ahmad
Jun 20, 2003·Apoptosis : an International Journal on Programmed Cell Death·T M CasertaT L Brown
Nov 7, 2003·Cancer·Michele FollenElliot K Fishman
Apr 7, 2004·Trends in Cell Biology·Mary C Abraham, Shai Shaham
Jan 27, 2005·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·M C Bell, R D Alvarez
Mar 8, 2005·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Miriam Ojeda OjedaWim A Buurman
May 4, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Linda E BrökerGiuseppe Giaccone
Aug 23, 2006·Cytotherapy·M A Franco-MolinaC Rodríguez-Padilla
Oct 11, 2008·Cell Death and Differentiation·G KroemerUNKNOWN Nomenclature Committee on Cell Death 2009
Dec 2, 2009·Cancer Biology & Therapy·Shi-Yong Sun
Mar 9, 2010·Immunopharmacology and Immunotoxicology·Moisés A Franco-MolinaCristina Rodríguez-Padilla
Jul 9, 2011·Cell Death and Differentiation·L CabonS A Susin
Nov 8, 2011·Methods in Molecular Biology·Jan M SuskiMariusz R Wieckowski
Dec 27, 2011·Journal of Experimental Botany·María Rodríguez-SerranoPilar S Testillano
Apr 20, 2012·The New England Journal of Medicine·Nicholas D JamesUNKNOWN BC2001 Investigators
Dec 27, 2012·The World Allergy Organization Journal·Esra BirbenOmer Kalayci
Jan 8, 2013·Journal of the National Cancer Institute·Silvia C Formenti, Sandra Demaria
Jan 9, 2013·Free Radical Biology & Medicine·Emiliano PanieriBoris Zhivotovsky
Apr 27, 2013·Nature Reviews. Immunology·Carl Nathan, Amy Cunningham-Bussel
Nov 30, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Arkadiusz GajekAgnieszka Marczak
Dec 19, 2013·Nature Chemical Biology·Scott J Dixon, Brent R Stockwell
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

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Methods Mentioned

BETA
fluorescence microscopy
flow cytometry
fluorescence
flow

Software Mentioned

FlowJo
GraphPad Prism
Image

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