Immunity induced with a Salmonella enterica serovar Enteritidis live vaccine is regulated by Th1-cell-dependent cellular and humoral effector mechanisms in susceptible BALB/c mice

Vaccine
Jörg LehmannGottfried Alber

Abstract

The objective of this study was to characterize the immune response induced by a live attenuated Salmonella Enteritidis (SE; ade(-)/his(-)) vaccine using an intraperitoneal immunization/challenge model in susceptible wild-type and cytokine-deficient BALB/c mice. In wild-type mice, inoculation of the SE live vaccine induced a protective immune response characterized by both cellular (production of interleukin(IL)-12 and interferon(IFN)-gamma, granuloma formation in liver and spleen, DTH response) and humoral effector mechanisms (high antigen-specific IgG2a titers). IL-12- and IL-4-deficient mice were immunized to study the individual roles of Th1 and Th2 cells, respectively. Protective immunity in wild-type mice required inoculation of >5 x 10(3)CFU of the attenuated live SE vaccine strain used. While IL-4-deficient mice developed a protective immune response similar to that found in wild-type mice, it was not possible to induce protective immunity in the highly susceptible IL-12-deficient mice due to severe disease symptoms and death following inoculation of the SE vaccine strain (doses >or=5 x 10(2)CFU were lethal for IL-12-deficient mice). Interestingly, persistence of the vaccine strain was observed in IL-4-deficient mice, i...Continue Reading

Citations

Nov 8, 2008·Journal of Occupational Medicine and Toxicology·Nasr Ya Hemdan
Jun 16, 2015·Frontiers in Cellular and Infection Microbiology·Zhanna A KtsoyanRustam I Aminov
Jun 1, 2014·Comparative Immunology, Microbiology and Infectious Diseases·Chetan V Jawale, John Hwa Lee
May 28, 2011·International Immunopharmacology·Rajeev Nagill, Sukhbir Kaur
Sep 24, 2004·Microbes and Infection·Esther von Stebut, Mark C Udey
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Silke M SchulzGottfried Alber

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