PMID: 7520465Sep 1, 1994Paper

Immunity to malaria elicited by hybrid hepatitis B virus core particles carrying circumsporozoite protein epitopes

The Journal of Experimental Medicine
F SchödelD R Milich

Abstract

The hepatitis B virus (HBV) nucleocapsid antigen (HBcAg) was investigated as a carrier moiety for the immunodominant circumsporozoite (CS) protein repeat epitopes of Plasmodium falciparum and the rodent malaria agent P. berghei. For this purpose hybrid genes coding for [NANP]4 (C75CS2) or [DP4NPN]2 (C75CS1) as internal inserts in HBcAg (between amino acids 75 and 81) were constructed and expressed in recombinant Salmonella typhimurium. The resulting hybrid HBcAg-CS polypeptides purified from S. typhimurium were particulate and displayed CS and HBc antigenicity, however, the HBc antigenicity was reduced compared to native recombinant HBcAg. Immunization of several mouse strains with HBcAg-CS1 and HBcAg-CS2 particles resulted in high titer, P.berghei- or P.falciparum-specific anti-CS antibodies representing all murine immunoglobulin G isotypes. The possible influence of carrier-specific immunosuppression was examined, and preexisting immunity to HBcAg did not significantly affect the immunogenicity of the CS epitopes within HBcAg-CS1 particles. Similarly, the choice of adjuvant did not significantly alter the immunogenicity of HBcAg-CS hybrid particles. Immunization in complete or incomplete Freund's adjuvant or alum resulted in ...Continue Reading

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Citations

Aug 1, 1997·Parasitology Today·S C Gilbert, A V Hill
Jun 19, 1998·Veterinary Microbiology·A BourgogneM Pépin
May 31, 1995·Annals of the New York Academy of Sciences·D R MilichF Schödel
Oct 18, 2005·Journal of Virology·Jean-Noel BillaudDavid Milich
Jun 16, 2004·Proceedings of the National Academy of Sciences of the United States of America·Martin L KoserMatthias J Schnell
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·T FehrR M Zinkernagel
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·P A KratzM Nassal
Dec 4, 2009·Amino Acids·Michiel Etienne JanssensYves Guisez
Jan 1, 1994·International Reviews of Immunology·F SchödelD Milich
Jun 5, 2007·Expert Review of Vaccines·Bryce Chackerian
Oct 30, 2009·Expert Review of Vaccines·David C WhitacreDavid R Milich
Nov 3, 2011·Expert Review of Vaccines·Luigi BuonaguroFranco M Buonaguro
Oct 7, 2010·Expert Review of Vaccines·António RoldãoPaula M Alves
Feb 3, 2005·European Journal of Immunology·Sandro PratoJosé Alejandro López
Apr 28, 2016·Journal of Nanobiotechnology·Susan ThraneAdam F Sander
Jan 6, 2017·Biomaterials·Izzat Fahimuddin Bin Mohamed SuffianKhuloud T Al-Jamal
Feb 19, 2013·Expert Review of Vaccines·Kenny RooseXavier Saelens
Jun 12, 2014·Biotechnology and Bioengineering·Mitchell TylerBryce Chackerian
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Alex J SpiceKaren M Polizzi
Jun 25, 2019·Frontiers in Immunology·Yun Shan GohLaurent Rénia
May 7, 2020·Journal of Molecular Biology·Virgile RatHugues de Rocquigny

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