Immunization against Egyptian Schistosoma mansoni infection by multivalent DNA vaccine

Acta Biochimica Et Biophysica Sinica
Mahmoud H RomeihMohamed A Saber

Abstract

The development of multivalent vaccines consisting of several antigens is a novel approach to creating broad-range protection against different parasite strains and parasite life cycle stages. We have previously confirmed that the schistosome Sm21.7 and SmFimbrin (SmFim) proteins could induce protection in mice. Therefore, this study aimed to construct the multivalent DNA vaccine Sm21.7-SmFim/pBudCE4.1 and evaluate its immune efficacy. The open reading frames of two Schistosoma mansoni genes, Sm21.7 and SmFim, were inserted into the eukaryotic expression plasmid pBudCE4.1 designed for the independent expression of two genes in mammalian cells. To evaluate the in vitro expression of the multivalent Sm21.7-SmFim/pBudCE4.1 DNA vaccine and its immunological effect in mice, the recombinant plasmid Sm21.7-SmFim/pBudCE4.1 was used to transfect 293T cells, and the expression of mRNA and proteins was examined using reverse transcription-polymerase chain reaction and Western blot analysis. Then the ability of Sm21.7-SmFim/pBudCE4.1 to protect against S. mansoni challenge infections was analyzed according to worm burden and egg reduction rates after vaccination of mice. Vaccinated mice showed a significant level of protection (56%), and a...Continue Reading

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Citations

May 3, 2014·Parasitology·Barbara Castro Pimentel FigueiredoSergio Costa Oliveira
Oct 21, 2011·Journal of Biomedicine & Biotechnology·Carina S PinheiroSergio C Oliveira
Aug 14, 2019·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Alaa Eldin M Labib El GendyNesma Atef Mohammed
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Nov 30, 2020·Vaccine·Joseane C de CastroRicardo T Fujiwara

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