Immunization against endogenous inhibin increases normal oocyte/embryo production in adult mice

Endocrine
Mohamed S MedanKazuyoshi Taya

Abstract

The objectives of the present study were to induce super-ovulation in adult mice by immunoneutralization of endogenous inhibin and to investigate embryo development in vivo and in vitro. Adult female mice of the ddY strain at 3 mo of age were superovulated with a single ip injection of inhibin antiserum (inhibin-AS; 50, 100, or 200 micro L) at 12:00 h on metestrus of the 4-d estrous cycle. The control group was treated with 100 micro L normal goat serum (control serum). Five mice were sacrificed every 6 h and blood samples were collected for hormonal assay. Individual female mice were bred in the afternoon of proestrus with individual fertile males. Embryos were collected at 08.00 h on d 2 post-coitus and cultured in KSOM solution. Treatment with inhibin-AS significantly increased the concentrations of plasma FSH. Furthermore, there was a significant elevation in plasma concentrations of estradiol-17beta and progesterone in the inhibin-immunized groups in comparison with controls. The superovulated oocytes that were fertilized normally in vivo were able to develop normally to blastocysts in vitro. The number of oocytes and blastocysts developed in animals treated with inhibin-AS was significantly higher than that for mice treat...Continue Reading

Citations

Jul 22, 2005·The Journal of Reproduction and Development·Harumichi IshigameKazuyoshi Taya
Jun 8, 2006·The Journal of Reproduction and Development·Kazuaki SasakiKazuyoshi Taya
Jan 14, 2005·The Journal of Reproduction and Development·Mohamed S MedanKazuyoshi Taya
Aug 11, 2011·Molecular and Cellular Endocrinology·Andrew N Shelling
Nov 18, 2015·Animal Reproduction Science·Leyan YanZhendan Shi
Jun 15, 2006·American Journal of Physiology. Endocrinology and Metabolism·Deenaz ZaidiGraham F Wagner
Nov 6, 2020·Reproduction in Domestic Animals = Zuchthygiene·Ruoxin JiaJianzhi Pan

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