Immunization of A4galt-deficient mice with glycosphingolipids from renal cell cancers resulted in the generation of anti-sulfoglycolipid monoclonal antibodies

Glycoconjugate Journal
Reiko AndoKoichi Furukawa

Abstract

In this study, we immunized Gb3/CD77 synthase gene (A4galt) knockout (KO) mice with glycosphingolipids (GSLs) extracted from 3 renal cell cancer (RCC) cell lines to raise monoclonal antibodies (mAbs) reactive with globo-series GSLs specifically expressed in RCCs. Although a number of mAbs reactive with globo-series GSLs were generated, they reacted with both RCC cell lines and normal kidney cells. When we analyzed recognized antigens by mAbs that were specifically reactive with RCC, but not with normal kidney cells at least on the cell surface, many of them turned out to be reactive with sulfoglycolipids. Eight out of 11 RCC-specific mAbs were reactive with SM2 alone, and the other 3 mAbs were more broadly reactive with sulfated glycolipids, i.e. SM3 and SM4 as well as SM2. In the immunohistochemistry, these anti-sulfoglycolipids mAbs showed RCC-specific reaction, with no or minimal reaction with adjacent normal tissues. Thus, immunization of A4galt KO mice with RCC-derived GSLs resulted in the generation of anti sulfated GSL mAbs, and these mAbs may be applicable for the therapeutics for RCC patients.

References

May 1, 1991·European Journal of Immunology·M MangeneyJ Wiels
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A N HoughtonL J Old
Aug 1, 1985·Journal of Immunogenetics·K KasaiS I Hakomori
Dec 1, 1981·In Vitro·R H Kennett
Sep 30, 1993·International Journal of Cancer. Journal International Du Cancer·F IncardonaC Legrand
Apr 1, 1996·Trends in Microbiology·C A Lingwood
Jul 18, 2000·Molecular Pathology : MP·P N NelsonP G Murray
Feb 15, 2005·Biochimica Et Biophysica Acta·Roger SandhoffHermann-Josef Gröne
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Olga KovbasnjukMark Donowitz
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zoran V PopovicHermann-Josef Gröne
Aug 9, 2008·Molecular Cancer Therapeutics·Thomas FalguièresKlaus-Peter Janssen
Jul 18, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Taro SugiyamaMasao Iwamori
Mar 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yuji KondoKoichi Furukawa

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Citations

Jul 25, 2019·Organic & Biomolecular Chemistry·Ingrid M E 't HartGeert-Jan Boons

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