PMID: 9423874Jan 10, 1998Paper

Immunization of the female genital tract with a DNA-based vaccine

Infection and Immunity
J B LivingstonD J Anderson

Abstract

Vaccines are being sought for contraception and the prevention of sexually transmitted diseases. However, progress is slow in this area largely because of lack of information on induction of protective immune responses in genital tract mucosa. In this study, we investigated whether in vivo transfection with a model DNA-based antigen delivered by gene gun technology would induce an antibody response detectable in vaginal secretions. Female rats were immunized with plasmids encoding human growth hormone (HGH) under the control of a cytomegalovirus promoter (pCMV/HGH) via vaginal mucosa (V), Peyer's patch (PP), and/or abdominal skin (S) routes. Localization of HGH in the target tissues demonstrated that all three sites can be transfected in vivo with pCMV/HGH. Vaginal tissues expressed roughly the same level of plasmid as skin. Antibodies to HGH were detectable in serum and vaginal secretions in rats immunized with pCMV/HGH. In the rats primed and boosted vaginally, vaginal immunoglobulin A (IgA) and IgG antibody titers to HGH were sustained for at least 14 weeks, whereas rats immunized via other routes and protocols (S/V, S/S, PP/PP, or PP/V) did not consistently sustain significant vaginal antibody titers beyond week 6. DNA-base...Continue Reading

References

Nov 1, 1979·Fertility and Sterility·S L Yang, G F Schumacher
Sep 1, 1985·British Journal of Obstetrics and Gynaecology·J N Edwards, H B Morris
Oct 1, 1968·American Journal of Obstetrics and Gynecology·M S YounesS A Bencosme
May 1, 1983·British Journal of Obstetrics and Gynaecology·H H MorrisD Y Mason
Aug 1, 1995·Immunity·D M Pardoll, A M Beckerleg
Jan 1, 1994·American Journal of Reproductive Immunology : AJRI·W H Kutteh, J Mestecky
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·E F FynanH L Robinson
Sep 1, 1996·The Journal of Infectious Diseases·J E HerrmannH L Robinson
Oct 1, 1996·Nature Medicine·C CondonL D Falo
Aug 1, 1996·Journal of Reproductive Immunology·D J Anderson
Jul 1, 1996·Emerging Infectious Diseases·R G Whalen
Sep 1, 1996·Baillière's Clinical Gastroenterology·A Panja, L Mayer
Dec 1, 1996·The Journal of Laboratory and Clinical Medicine·R M Steinman
Aug 1, 1997·The Journal of Infectious Diseases·H L RobinsonR G Webster

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Citations

Mar 24, 2007·Annual Review of Immunology·Akiko Iwasaki
Apr 25, 2008·Current Protocols in Immunology·H L Robinson, T M Pertmer
Aug 7, 2012·Expert Opinion on Therapeutic Patents·Pallavi Bassi, Gurpreet Kaur
Oct 18, 2014·Cellular and Molecular Life Sciences : CMLS·Jill M Steinbach
May 25, 1999·Microbiology and Immunology·S SasakiK Okuda
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·F M OrsonM A Barry
Mar 8, 2008·Expert Review of Vaccines·Shan LuJill M Grimes-Serrano
Mar 21, 2002·Immunology and Cell Biology·A K Sharma, G K Khuller
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·David AlvarezManel Jordana
Jun 21, 2011·Genetic Vaccines and Therapy·Mohammad Feraz Ahsan, Milind M Gore
Jun 14, 2000·Journal of Virology·B I Loehr van Drunen Littel-van den Hurk
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P HärleD J Carr
Jun 3, 2000·Annual Review of Immunology·S GurunathanR A Seder
Sep 11, 2010·Nature Reviews. Immunology·Akiko Iwasaki
Jan 31, 2004·American Journal of Reproductive Immunology : AJRI·Christine M GockelKenneth W Beagley
Feb 11, 2014·Current Protocols in Microbiology·Shixia Wang, Shan Lu
Sep 6, 2000·Veterinary Immunology and Immunopathology·L A Babiuk van Drunnen Littel-van den Hur
Jul 7, 1999·Journal of Reproductive Immunology·J Frayne, L Hall
Dec 28, 1999·Microbes and Infection·M J McCluskie, H L Davis
Jul 3, 2021·Vaccines·Joseph HokelloMudit Tyagi

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