Immunization with a plasmid DNA vaccine encoding the N-terminus of Insulin like Growth Factor Binding Protein-2 in advanced ovarian cancer leads to high level Type I immune responses.

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Denise L CecilMary L Disis


Cancer vaccines targeting nonmutated proteins elicit limited Type I T-cell responses and can generate T-regulatory and Type II T-cells. Class II epitopes that selectively elicit Type I or Type II cytokines can be identified in nonmutated cancer associated proteins. In mice, a T-helper I selective IGFBP-2 N-terminus vaccine generated high levels of IFN-Ɣ secreting T-cells, no regulatory T-cells and significant anti-tumor activity. We conducted a Phase I trial of Th1 selective IGFBP-2 vaccination in patients with advanced ovarian cancer. Twenty-five patients were enrolled. The IGFBP-2 N-terminus plasmid-based vaccine was administered monthly for three months. Toxicity was graded by NCI criteria and antigen specific T-cells measured by IFN-Ɣ/IL-10 ELISPOT. T-cell diversity and phenotype were assessed. The vaccine was well tolerated, 99% of adverse events graded 1 or 2, and generated high levels of IGFBP-2 IFN-Ɣ secreting T-cells in 50% of patients. Both Tbet+ CD4 (p=0.04) and CD8 (p=0.007) T-cells were significantly increased in immunized patients. There was no increase in GATA3+ CD4 or CD8, IGFBP-2 IL-10 secreting T-cells or T-regulatory cells. A significant increase in T-cell clonality occurred in immunized patients (p=0.03, pre...Continue Reading

Related Concepts

Related Feeds

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Cancer Vaccines

Cancer vaccines are vaccines that either treat existing cancer or prevent development of a cancer.

© 2021 Meta ULC. All rights reserved