Immunization with an autotransporter protein of Orientia tsutsugamushi provides protective immunity against scrub typhus

PLoS Neglected Tropical Diseases
Na-Young HaNam-Hyuk Cho

Abstract

Scrub typhus is an acute febrile disease caused by Orientia tsutsugamushi infection. Recently, the rapid increase of scrub typhus incidence in several countries within the endemic region has become a serious public health issue. Despite the wide range of preventative approaches that have been attempted in the past 70 years, all have failed to develop an effective prophylactic vaccine. Currently, the selection of the proper antigens is one of the critical barriers to generating cross-protective immunity against antigenically-variable strains of O. tsutsugamushi. We examined the potential role of ScaA protein, an autotransporter protein of O. tsutsugamushi, in bacterial pathogenesis and evaluated the protective attributes of ScaA immunization in lethal O. tsutsugamushi infection in mice. Our findings demonstrate that ScaA functions as a bacterial adhesion factor, and anti-ScaA antibody significantly neutralizes bacterial infection of host cells. In addition, immunization with ScaA not only provides protective immunity against lethal challenges with the homologous strain, but also confers significant protection against heterologous strains when combined with TSA56, a major outer membrane protein of O. tsutsugamushi. Immunization o...Continue Reading

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Citations

Feb 2, 2018·Clinical Microbiology Reviews·Fabián E DíazAlexis M Kalergis
Sep 21, 2018·PLoS Neglected Tropical Diseases·Munegowda C KoralurGregory A Dasch
Feb 3, 2016·The Journal of Microbiology·Sunyoung BangNam-Hyuk Cho
Apr 18, 2018·Advanced Healthcare Materials·Chisong LeeHyungil Jung
Oct 23, 2020·PLoS Neglected Tropical Diseases·Anke Osterloh
Dec 19, 2020·International Journal of Medical Microbiology : IJMM·Anwesha Banerjee, Smita Kulkarni
Nov 27, 2019·Veterinary Microbiology·Sahlu AyalewAnthony W Confer
Oct 29, 2021·Frontiers in Immunology·Chih-Chi LiaoYu-Chan Chao

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Datasets Mentioned

BETA
AY836148.1

Methods Mentioned

BETA
PCR
ELISA
confocal microscopy
flow cytometry
fluorescence microscopy
fluorescence-activated cell sorter
ELISAs

Software Mentioned

Graph Pad Prism
Clustal W
MEGA6
Olympus Fluoview
Graph
- Pad
GraphPad Prism
Matrix Global Alignment Tool ( MatGAT )
Fluoview
Tandem Repeat Finder

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