PMID: 8592095Jan 1, 1996Paper

Immunization with cholesterol-rich liposomes induces anti-cholesterol antibodies and reduces diet-induced hypercholesterolemia and plaque formation

The Journal of Laboratory and Clinical Medicine
C R AlvingJ F Cornhill

Abstract

Immunization of rabbits with a protein-free formulation consisting of liposomes containing 71% cholesterol and lipid A as an adjuvant induced anticholesterol antibodies that caused complement-dependent lysis of liposomes lacking lipid A. The antibodies, immunoglobulin G (IgG) and immunoglobulin M (IgM), also recognized nonoxidized crystalline cholesterol as an antigen by enzyme-linked immunosorbent assay (ELISA). The effects of immunization against cholesterol on elevations in serum cholesterol and development of atherosclerosis were examined in rabbits fed a diet containing 0.5% to 1.0% cholesterol. Although the mean serum cholesterol level, mainly in the form of very-low-density lipoprotein cholesterol, rose as much as 60-fold in the nonimmunized rabbits, the elevation was significantly less--as much as 35% lower--in the immunized rabbits. Elevation of serum cholesterol was accompanied by an apparent drop in the level of antibodies on initiating the diet, followed by a rebound on stopping the diet, thus suggesting that the antibodies were adsorbed to cholesterol that was present in circulating lipoproteins. When lipoprotein fractions--composed of either very-low-density and intermediate-density lipoproteins derived from chole...Continue Reading

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Citations

Jan 1, 1996·Immunologic Research·J GeorgeY Shoenfeld
Oct 24, 2000·Journal of Immunological Methods·G R MatyasC R Alving
Sep 11, 2003·Autoimmunity Reviews·Anna Horváth, Adrienn Bíró
Feb 28, 2009·Pharmaceutical Research·Wim JiskootHuub Schellekens
Jun 26, 2009·Clinical Reviews in Allergy & Immunology·Jozélio Freire de CarvalhoYehuda Shoenfeld
Mar 10, 2007·World Journal of Surgery·Charles W Rittershaus
Apr 23, 2004·Expert Opinion on Biological Therapy·Xinghua Zhou, Göran K Hansson
Jan 17, 2016·Toxicology and Applied Pharmacology·Anna N Ilinskaya, Marina A Dobrovolskaia
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Oct 16, 2004·Expert Review of Vaccines·Dror Harats, Jacob George
Nov 3, 2009·Lupus·J F CarvalhoY Shoenfeld
Apr 24, 2014·Therapeutic Advances in Vaccines·Kuang-Yuh Chyu, Prediman K Shah
Sep 6, 2006·Journal of Liposome Research·Carl R Alving
Aug 23, 2008·Journal of Applied Physiology·Lakshmi SanthanamDan E Berkowitz
Sep 17, 2013·Journal of Cardiovascular Pharmacology and Therapeutics·Kuang-Yuh Chyu, Prediman K Shah
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