Immunization With Fc-Based Recombinant Epstein-Barr Virus gp350 Elicits Potent Neutralizing Humoral Immune Response in a BALB/c Mice Model

Frontiers in Immunology
Bingchun ZhaoYixin Zeng

Abstract

Epstein-Barr virus (EBV) was the first human virus proved to be closely associated with tumor development, such as lymphoma, nasopharyngeal carcinoma, and EBV-associated gastric carcinoma. Despite many efforts to develop prophylactic vaccines against EBV infection and diseases, no candidates have succeeded in effectively blocking EBV infection in clinical trials. Previous investigations showed that EBV gp350 plays a pivotal role in the infection of B-lymphocytes. Nevertheless, using monomeric gp350 proteins as antigens has not been effective in preventing infection. Multimeric forms of the antigen are more potently immunogenic than monomers; however, the multimerization elements used in previous constructs are not approved for human clinical trials. To prepare a much-needed EBV prophylactic vaccine that is potent, safe, and applicable, we constructed an Fc-based form of gp350 to serve as a dimeric antigen. Here, we show that the Fc-based gp350 antigen exhibits dramatically enhanced immunogenicity compared with wild-type gp350 protein. The complete or partial gp350 ectodomain was fused with the mouse IgG2a Fc domain. Fusion with the Fc domain did not impair gp350 folding, binding to a conformation-dependent neutralizing antibody...Continue Reading

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Citations

Nov 14, 2018·Immunopharmacology and Immunotoxicology·Zhen XiangQing-Yuan Zhang
Aug 26, 2020·Leukemia & Lymphoma·Zijun Y Xu-Monette, Ken H Young
Mar 14, 2019·F1000Research·Aaron GregsonDavid L Selwood
May 6, 2020·Cellular and Molecular Life Sciences : CMLS·Julia RühlChristian Münz
May 30, 2021·Journal of Immunological Methods·Etienne FrumencePhilippe Gasque
Jul 10, 2021·Frontiers in Microbiology·Vincent Jean-PierreRaphaële Germi
Jun 26, 2021·Frontiers in Immunology·Cong SunMu-Sheng Zeng

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Methods Mentioned

BETA
glycosylation
PCR
dot blot
transfection
size exclusion chromatography
electrophoresis
sedation
ELISA
chip
FACS

Software Mentioned

GraphPad Prism
Sedfit

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