Immunization with high-molecular-weight adhesion proteins of nontypeable Haemophilus influenzae modifies experimental otitis media in chinchillas.

Infection and Immunity
S J Barenkamp

Abstract

Prevention of nontypeable Haemophilus influenzae otitis media by vaccination is an important health care goal. Proteins important in bacterial adherence deserve consideration as potential vaccine candidates. Two colleagues and I previously identified a family of immunogenic high-molecular-weight proteins important in adherence of nontypeable H. influenzae to human epithelial cells (J.W. St. Geme III, S. Falkow, and S.J. Barenkamp, Proc. Natl. Acad. Sci. USA, 90:2875-2879, 1993). In the work described here, I determined whether immunization with two such adherence proteins, HMW1 and HMW2, purified from prototype nontypeable Haemophilus strain 12, would modify the course of experimental otitis media caused by the homologous strain. Chinchillas received three monthly subcutaneous injections with 40 microgram of an HMW1/HMW2 protein mixture in Freud's adjuvant. One month after the last injection, animals were challenged by intrabullar inoculation with 300 CFU of nontypeable H. influenzae 12. Infection developed in five of five control animals versus 5 of 10 immunized animals (P = 0.08, Fisher exact, one-tailed). Among infected animals, bacterial counts in middle ear fluid specimens 7 days postchallenge were significantly greater in...Continue Reading

References

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Citations

Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DawidJ W St Geme
Oct 26, 1999·The Pediatric Infectious Disease Journal·S I Pelton, J O Klein
Jun 15, 2006·Molecular Microbiology·Amy Z BuscherJoseph W St Geme
Jul 16, 2008·The Journal of Biological Chemistry·Julia GrossJoseph W St Geme
May 29, 2009·Clinical and Vaccine Immunology : CVI·Linda E Winter, Stephen J Barenkamp
Mar 20, 2015·Clinical and Vaccine Immunology : CVI·Timothy F Murphy
Feb 20, 2016·Expert Review of Vaccines·M Nadeem KhanMichael E Pichichero
Jan 31, 2012·Applied and Environmental Microbiology·Makrina TotsikaMark A Schembri
Jun 9, 1998·Annals of the New York Academy of Sciences·G S Giebink
May 23, 2000·The Pediatric Infectious Disease Journal·J Eskola, T Kilpi
May 26, 2017·Critical Reviews in Microbiology·Krishna Bajee SriramAllan W Cripps
Jan 26, 2008·Animal Health Research Reviews·S M DaboA W Confer
Oct 24, 1998·Infection and Immunity·J R Gilsdorf
Nov 28, 2019·Journal of Proteome Research·Danila Elango, Benjamin L Schulz
Sep 16, 1999·Journal of Biotechnology·J Kyd, A Cripps
Aug 17, 2002·Current Opinion in Immunology·Allan W CrippsJennelle Kyd
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A R FoxwellA W Cripps

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