Immunization with inflammatory proteome of Brugia malayi adult worm induces a Th1/Th2-immune response and confers protection against the filarial infection

Vaccine
M K SahooP K Murthy

Abstract

Mastomys coucha and jirds (Meriones unguiculatus) were immunized with four cytokine-stimulating SDS-PAGE resolved fractions F5 (68-84 kDa), F6 (54-68 kDa), F10 (38-42 kDa) and F14 (20-28 kDa) of Brugia malayi adult worm to determine which of these fractions has the potential to influence the establishment of subsequently introduced B. malayi infection in the animals. The proteins in the fractions were analyzed by 2DE and MALDI-TOF. Immunization with F6 suppressed the establishment of third stage larva (L(3)) initiated infection in M. coucha (64%; P<0.01) and jird (42%; P<0.01). Survival of intraperitoneally implanted adult worms in M. coucha was lowered by F6 (72%; P<0.01) and F14 (66%; P<0.05) but not by F5 and F10. Immunization with F6 intensely upregulated both Th1 (IFN-gamma, TNF-alpha, IL-1 beta, IL-2, IL-6, IgG1, IgG2a and lymphoproliferation) and Th2 (IgG2b and IL-10) responses and NO release. Immunostimulatory proteins HSP60, intermediate filament protein, and translation elongation factor EF-2 were identified in F6 fraction by 2DE and MALDI. The findings suggest that F6 protects the host from the parasite via Th1/Th2 type responses and thus holds promise for development as a vaccine.

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Citations

Jul 5, 2013·Clinical Microbiology Reviews·C Paul MorrisEdward Mitre
Oct 23, 2013·PloS One·Gajalakshmi DakshinamoorthyRamaswamy Kalyanasundaram
Jun 28, 2015·Experimental Parasitology·Anna M van der Ree, Francisca Mutapi
Oct 6, 2012·Vaccine·Gajalakshmi DakshinamoorthyRamaswamy Kalyanasundaram
Jul 21, 2011·Asian Pacific Journal of Tropical Medicine·Shiv Kumar VermaPuvvada Kalpana Murthy
Apr 8, 2015·PLoS Neglected Tropical Diseases·Vijayan KamalakannanRangarajan Badri Narayanan
Jun 28, 2019·Current Topics in Medicinal Chemistry·Puvvada Kalpana Murthy
Jun 28, 2018·Parasitology Research·Richa VermaPuvvada Kalpana Murthy

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