Immunization with Leishmania major exogenous antigens protects susceptible BALB/c mice against challenge infection with L. major

Infection and Immunity
Willy K TonuiRichard G Titus

Abstract

The potential of Leishmania major culture-derived soluble exogenous antigens (SEAgs) to induce a protective response in susceptible BALB/c mice challenged with L. major promastigotes was investigated. Groups of BALB/c mice were immunized with L. major SEAgs alone, L. major SEAgs coadministered with either alum (aluminum hydroxide gel) or recombinant murine interleukin-12 (rmIL-12), L. major SEAgs coadministered with both alum and rmIL-12, and L. major SEAgs coadministered with Montanide ISA 720. Importantly and surprisingly, the greatest and most consistent protection against challenge with L. major was seen in mice immunized with L. major SEAgs alone, in the absence of any adjuvant. Mice immunized with L. major SEAgs had significantly smaller lesions that at times contained more than 100-fold fewer parasites. When lymphoid cells from L. major SEAg-immunized mice were stimulated with leishmanial antigen in vitro, they proliferated and secreted a mixed profile of type 1 and type 2 cytokines. Finally, analyses with Western blot analyses and antibodies against three surface-expressed and secreted molecules of L. major (lipophosphoglycan, gp46/M2/PSA-2, and gp63) revealed that two of these molecules are present in L. major SEAgs, l...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·F P HeinzelR M Locksley
Mar 30, 1984·Science·D L Sacks, P V Perkins
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·R G TitusS M Beverley
Jan 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D J MatthewsA N McKenzie
Apr 9, 2001·Clinical Microbiology Reviews·E Handman
Aug 20, 2002·Vaccine·Norman W BaylorPaul Richman
Oct 3, 2002·American Journal of Clinical Dermatology·Peter C Melby
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nancy Noben-TrauthDavid L Sacks
Aug 6, 2003·Current Opinion in Immunology·Steven G Reed, Antonio Campos-Neto
Jan 9, 2004·Expert Review of Vaccines·Cláudia BrodskynManoel Barral-Netto
Feb 1, 1997·Parasitology Today·H C LimaR G Titus

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Citations

Feb 18, 2011·Archives of Dermatological Research·J A O'DalyN R Indriago
Sep 2, 2011·Archives of Dermatological Research·Luiz Felipe Domingues PasseroGabriela Santos-Gomes
Feb 6, 2007·Parasitology·L KedzierskiE Handman
Aug 15, 2009·Nature Reviews. Microbiology·Alan R Hauser
Nov 22, 2005·Infection and Immunity·Rhoel R Dinglasan, Marcelo Jacobs-Lorena
Jan 1, 2009·African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM·Peter Kamau NgureWilly Kiprotich Tonui
Feb 11, 2010·Journal of Biomedicine & Biotechnology·Rosa M CorralesDenis Sereno
Aug 22, 2007·Journal of Biomedicine & Biotechnology·Nuno SantarémAnabela Cordeiro-da-Silva
May 22, 2010·Trends in Parasitology·Alexandre B ReisJavier Moreno
Apr 19, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·F Z GuerfaliD Laouini
Oct 11, 2005·Experimental Parasitology·A R KhabiriS R Nadaf
Apr 6, 2013·Journal of Proteomics·Nuno SantarémMarc Ouellette
Sep 29, 2011·Parasites & Vectors·Albin FontaineLionel Almeras
Mar 13, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Rachel Bras-GonçalvesJean-Loup Lemesre
May 28, 2011·International Immunopharmacology·Rajeev Nagill, Sukhbir Kaur
Oct 8, 2009·FEMS Immunology and Medical Microbiology·Rosa Milagros CorralesFrançoise Mathieu-Daudé
Sep 30, 2008·PLoS Neglected Tropical Diseases·Vera VolfovaPetr Volf
Jun 21, 2006·Trends in Parasitology·J Santiago MejiaRichard G Titus
Oct 9, 2004·Trends in Parasitology·J Santiago MejiaRichard G Titus
May 14, 2010·Revista Do Instituto De Medicina Tropical De São Paulo·Joshua M MutisoEvans Taracha
May 3, 2018·Expert Review of Proteomics·Shyam Sundar, Bhawana Singh
Jan 10, 2020·Expert Opinion on Drug Delivery·Anis AskarizadehAli Khamesipour
May 22, 2019·Acta Parasitologica·Mehdi NamavariMohammad Asadpour
Oct 31, 2017·Asian Pacific Journal of Tropical Medicine·Adil M AllahverdiyevZeynep Akdeste
May 14, 2021·Frontiers in Cellular and Infection Microbiology·Tanvir BamraPradeep Das
Jan 1, 2010·Journal of Biomedical Research·Joshua M MutisoMichael M Gicheru

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