Immunization with live attenuated influenza viruses that express altered NS1 proteins results in potent and protective memory CD8+ T-cell responses.

Journal of Virology
Scott N MuellerRafi Ahmed

Abstract

The generation of vaccines that induce long-lived protective immunity against influenza virus infections remains a challenging goal. Ideally, vaccines should elicit effective humoral and cellular immunity to protect an individual from infection or disease. Cross-reactive T- and B-cell responses that are elicited by live virus infections may provide such broad protection. Optimal induction of T-cell responses involves the action of type I interferons (IFN-I). Influenza virus expressed nonstructural protein 1 (NS1) functions as an inhibitor of IFN-I and promotes viral growth. We wanted to examine the priming of CD8(+) T-cell responses to influenza virus in the absence of this inhibition of IFN-I production. We generated recombinant mouse-adapted influenza A/PR/8/34 viruses with NS1 truncations and/or deletions that also express the gp33-41 epitope from lymphocytic choriomeningitis virus. Intranasal infection of mice with the attenuated viruses primed long-lived T- and B-cell responses despite significantly reduced viral replication in the lungs compared to wild-type virus. Antigen-specific CD8(+) T cells expanded upon rechallenge and generated increased protective memory T-cell populations after boosting. These results show that ...Continue Reading

References

Mar 1, 1992·Microbiological Reviews·R G WebsterY Kawaoka
Jun 1, 1990·The Journal of General Virology·E HatadaR Fukuda
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·J TalonP Palese
May 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·E HoffmannR G Webster
Feb 4, 2003·Nature Immunology·E John WherryRafi Ahmed
May 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Richard J WebbyStephen J Turner
Aug 6, 2003·Current Opinion in Immunology·David L Woodland
Feb 3, 2004·Immunology and Allergy Clinics of North America·Robert B Belshe, Paul M Mendelman
Apr 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Julie M CurtsingerMatthew F Mescher
Jun 14, 2005·Nature Medicine·Vladimir P BadovinacJohn T Harty
Jun 16, 2005·Journal of Virology·Michelle QuinlivanPeter Palese
Sep 1, 2005·The Journal of Experimental Medicine·Ganesh A KolumamKaja Murali-Krishna
Jan 18, 2006·Immunity·Kanta SubbaraoAnthony S Fauci
Mar 7, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Colin Havenar-DaughtonKaja Murali-Krishna
Apr 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ji-Young Min, Robert M Krug
Jun 16, 2006·Journal of Virology·Ana Fernandez-SesmaThomas M Moran
Sep 12, 2006·Proceedings of the National Academy of Sciences of the United States of America·Benjamin G HaleRichard E Randall
Oct 14, 2006·Science·Andreas PichlmairCaetano Reis e Sousa
Mar 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·David JacksonRobert A Lamb
Sep 18, 2008·The Journal of General Virology·Benjamin G HaleDavid Jackson
Feb 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Carole R BaskinMichael G Katze
Mar 25, 2009·Immunology and Cell Biology·Lorena E Brown, Anne Kelso
May 13, 2009·Science·Christophe FraserUNKNOWN WHO Rapid Pandemic Assessment Collaboration
May 16, 2009·Nature·Declan Butler
May 21, 2009·Cell Host & Microbe·Michaela Ulrike GackAdolfo García-Sastre

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Citations

Nov 8, 2012·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Christopher P Malinoski, Philip I Marcus
Oct 19, 2011·Journal of Biomedicine & Biotechnology·Marine L B HillaireGuus F Rimmelzwaan
Oct 27, 2014·Nature Immunology·Ronald A BackerDerk Amsen
Oct 31, 2015·American Journal of Respiratory and Critical Care Medicine·Richard T McKendryTom M A Wilkinson
Oct 1, 2010·The Journal of General Virology·Marcin P WalkiewiczDaniel A Engel
Apr 13, 2013·The Journal of General Virology·Julie M FoxRalph A Tripp
Oct 12, 2010·Vaccine·Bin ZhouDavid E Wentworth
Aug 9, 2011·Vaccine·Guus F RimmelzwaanAlbert D M E Osterhaus
Jan 8, 2011·Vaccine·Kristin ScheibleDavid J Topham
Jul 20, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Scott N MuellerRafi Ahmed
Jun 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·José V Pérez-GirónCésar Muñoz-Fontela
Oct 8, 2020·Pathogens·Wenzhuo HaoShitao Li

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