Immunization with outer membrane protein P6 from nontypeable Haemophilus influenzae induces bactericidal antibody and affords protection in the chinchilla model of otitis media.

Infection and Immunity
T F DeMariaE R Leake

Abstract

The role of nontypeable Haemophilus influenzae (NTHi) outer membrane protein (OMP) P6 in the pathogenesis of otitis media (OM) has not been defined. OMPs, fimbriae, pili, and lipooligosaccharide are several types of surface antigens of NTHi that are currently being evaluated as potential vaccine candidates. P6 is antigenically conserved among both nontypeable and type b H. influenzae strains and elicits bactericidal as well as protective antibodies; however, initial evaluation of a vaccine mixture of P6 combined with other NTHi OMPs failed to induce bactericidal antibody or protection in the chinchilla model of OM. We undertook an assessment of the ability of immunization with isolated P6 lipoprotein alone to confer protection. Chinchillas were immunized with P6 and challenged 10 days after the final immunization with either 3 x 10(3) CFU of NTHi delivered directly into the middle ear to induce OM or 5 x 10(8) CFU of NTHi delivered intranasally to establish nasopharyngeal colonization. All immunized animals responded with elevated serum titers of anti-P6 antibody, which also demonstrated bactericidal activity against homologous as well as a heterologous NTHi isolate. By 14 days post-transbullar challenge, the number of chinchil...Continue Reading

References

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Citations

May 23, 2000·The Pediatric Infectious Disease Journal·J Eskola, T Kilpi
Apr 23, 2004·Infection and Immunity·Robert S MunsonLauren O Bakaletz
May 8, 2004·The Pediatric Infectious Disease Journal·Stephen J Barenkamp
May 29, 2009·Clinical and Vaccine Immunology : CVI·Linda E Winter, Stephen J Barenkamp
Mar 22, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Paul WebsterJoseph W St Geme
Nov 19, 2015·Frontiers in Microbiology·Shivani GoolabMichael C Crampton
Apr 2, 1998·Pediatric Annals·E E Adderson
Sep 23, 2006·Expert Review of Vaccines·Allan W Cripps, Diana C Otczyk
May 21, 2013·Journal of Bacteriology·Lea Vacca MichelMichael E Pichichero
Feb 20, 2016·Expert Review of Vaccines·M Nadeem KhanMichael E Pichichero
Aug 24, 2018·Journal of Medical Microbiology·Lea Vacca MichelMichael E Pichichero
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A R FoxwellA W Cripps

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