Abstract
An effective vaccine for severe acute respiratory syndrome (SARS) will probably require the generation and maintenance of both humoral and cellular immune responses. It has been reported that after natural infection in humans and immunization in animals with SARS-CoV vaccine, antibody is produced and persistent for a long period of time. In the present study, mice were immunized i.m. with SARS-CoV S DNA vaccine, and three different methods (ELISA, ELISPOT and FACS) were used to evaluate the immune responses when the cells were stimulated in vitro with a pool of peptides overlapping entire SARS spike protein. The results show that prime-immunization with SARS-CoV S DNA vaccine can induce both CD4(+) and CD8(+) T cell responses. Boosting with the same vaccine enhances CD4(+) and CD8(+) T cell responses in both lymphoid and nonlymphoid organs and were persistent over two months. The SARS-CoV S-specific CD4(+) and CD8(+) T cells were CD62L(-), a marker for memory cells, and -30 to 50% of the cells expressed IL-7Ralpha (CD127), a marker for the capacity of effector cells to develop into memory cells. In addition, immunization with the DNA vaccine elicited high levels of antibody production. Taken together, these data demonstrate tha...Continue Reading
References
Aug 13, 2002·Nature Immunology·Chang-You WuRobert A Seder
Feb 4, 2003·Nature Immunology·E John WherryRafi Ahmed
Feb 21, 2003·Nature·Edith M JanssenStephen P Schoenberger
Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
May 6, 2003·Science·Paul A RotaWilliam J Bellini
Aug 28, 2003·Nature Immunology·Robert A Seder, Rafi Ahmed
Nov 20, 2003·Nature Immunology·Susan M KaechRafi Ahmed
Dec 10, 2003·The Journal of Experimental Medicine·Robyn M KondrackLinda M Bradley
Dec 19, 2003·The New England Journal of Medicine·Joseph S M PeirisKlaus Stöhr
Apr 2, 2004·Nature·Zhi-Yong YangGary J Nabel
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Himani BishtBernard Moss
May 8, 2004·Journal of Clinical Microbiology·Patrick C Y WooKwok-yung Yuen
May 28, 2004·Journal of Virology·Heike HofmannStefan Pöhlmann
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ursula J BuchholzPeter L Collins
Jun 29, 2004·Lancet·Alexander BukreyevPeter L Collins
Jul 6, 2004·Immunological Reviews·Anne C Moore, Adrian V S Hill
Jul 6, 2004·Immunological Reviews·Marie J EstcourtTomás Hanke
Jul 13, 2004·Nature Medicine·Elisabetta TraggiaiAntonio Lanzavecchia
Apr 13, 2005·Virology·Yan ZhiJames M Wilson
Jun 29, 2005·The Journal of Experimental Medicine·Alan D RobertsDavid L Woodland
Jul 12, 2005·Vaccine·Alexander N ZakhartchoukLorne A Babiuk
Jul 27, 2005·Virology·Sagar U KapadiaAnjeanette Roberts
Nov 22, 2005·Drugs·Ian M Orme
Citations
Jun 16, 2009·Archives of Virology·Yan-Ying FanChang-You Wu
Jul 3, 2009·Journal of Virology·Aaruni KhanolkarJohn T Harty
Apr 9, 2010·Viral Immunology·Lanying DuShibo Jiang
Aug 2, 2007·DNA and Cell Biology·Alexander N ZakhartchoukLorne A Babiuk
Dec 3, 2009·Viral Immunology·Youmin KangGuoxing Zheng
Aug 17, 2010·Vaccine·Kai ZhaoChangyou Wu
Jan 21, 2010·Biochemical and Biophysical Research Communications·S S A AnE Welker
Oct 2, 2007·Molecular Immunology·Bingke BaiHanzhong Wang
May 29, 2009·Journal of Medical Virology·W P PohVincent T K Chow
Aug 17, 2012·Human Vaccines & Immunotherapeutics·Shih-Min WangChing-Chuan Liu
Feb 25, 2014·Vaccine·Cuiqing MaShibo Jiang
Aug 18, 2009·Virology·Lanying DuShibo Jiang
Jul 31, 2007·Biochemical and Biophysical Research Communications·Rui MaChang-you Wu
Aug 22, 2007·Vaccine·Jun HuangChangyou Wu
Jan 1, 2012·Cells·Stefanie KuertenPaul V Lehmann
May 15, 2007·Clinical and Vaccine Immunology : CVI·Hui HuHanzhong Wang
Nov 7, 2008·Clinical and Vaccine Immunology : CVI·Baojing LuHanzhong Wang
Mar 4, 2011·Scandinavian Journal of Infectious Diseases·Lifeng LiuTaisheng Li
Aug 14, 2020·Human Vaccines & Immunotherapeutics·Mohamad Hesam ShahrajabianQi Cheng
Sep 1, 2012·Emerging Microbes & Infections·Hsueh-Ling Janice OhYee-Joo Tan
Jun 18, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aaruni KhanolkarJohn T Harty
Feb 3, 2021·International Journal of Molecular Sciences·Nicholas A Wong, Milton H Saier
Jan 12, 2021·Frontiers in Immunology·Jing ZhaoQiwei Zhang
Jan 15, 2021·Journal of Virology·Eunok LeeSarah Palmer
Aug 7, 2020·Diabetes & Metabolic Syndrome·Kirtikumar C BadgujarShamkant B Badgujar