Immunization With Skp Delivered on Outer Membrane Vesicles Protects Mice Against Enterotoxigenic Escherichia coli Challenge

Frontiers in Cellular and Infection Microbiology
Michael P HaysPhilip R Hardwidge

Abstract

Outer membrane vesicles (OMVs) are promising vaccine components because they combine antigen and adjuvant in a single formulation. Detoxified Salmonella enterica strains that express penta-acylated lipid A retain OMV immunogenicity but with reduced reactogenicity. We have previously shown that a recombinant form of the enterotoxigenic Escherichia coli (ETEC) 17 kilodalton protein (Skp) protects mice in a pulmonary challenge model, when fused to the glutathione-S-transferase (GST) epitope and combined with cholera toxin. Here we compared directly the efficacy of expressing Skp in detoxified Salmonella OMVs to GST-Skp for their ability to protect mice against ETEC challenge. We observed that the display of Skp on OMVs, in the absence of exogenous adjuvant, protects the mice as well as the recombinant GST-Skp with adjuvant, showing that we can achieve protection when antigen and adjuvant are administered as a single formulation. Collectively, these data demonstrate the utility of using OMVs for the expression and display of antigens for use in vaccine development and validate previously published work demonstrating that immunization with Skp is efficacious in protecting mice against ETEC challenge.

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Citations

Oct 16, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xinpeng JiangDi Liu
Jun 2, 2020·Frontiers in Microbiology·H Bart van den Berg van SaparoeaWouter S P Jong
Mar 27, 2020·Frontiers in Cellular and Infection Microbiology·Christian Rueter, Martina Bielaszewska
Oct 23, 2020·Science Advances·Guillaume MasSebastian Hiller
Jan 27, 2019·Microbiology Spectrum·Jessica D CecilAnne Marie Krachler

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Methods Mentioned

BETA
PCR

Software Mentioned

Quantity One

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