Immuno-characterisation of neuroendocrine cells of the rat thymus gland in vitro and in vivo

Cell and Tissue Research
C A BothamM D Kendall

Abstract

Primary cell cultures and organ fragments of rat thymus were characterised by use of a panel of antibodies raised against the neural adhesion molecule L1, tyrosine hydroxylase, protein gene product 9.5, nerve growth factor, calcitonin gene-related peptide, glial fibrillary acidic protein, vimentin, pan-cytokeratin, a ganglioside of neural crest and neuroendocrine cells (A2B5), and thymulin (4 beta). Immunoreactivity for neural markers only was identified in a single morphology (nerve-like) of cell in culture and throughout the adult thymus as fine, tortuous staining. Immunoreactivity for endocrine markers only was identified in polygonal epithelial-like cells in culture, throughout viable tissue in fragment culture and in the subcapsular cortex of the adult thymus. Immunoreactivity for both endocrine and neural markers was identified in three distinct morphologies in cell culture: elongate, spherical, and stellate. Similar results were observed in the mitotic periphery of the cultured fragments and in the medulla and cortico-medullary junction of the adult thymus. Cells with immunoreactivity to A2B5 were present in primary and fragment cultures and occurred throughout the adult thymus. The apparent diversity of cell immunoreact...Continue Reading

Citations

Mar 25, 2004·Trends in Immunology·Enrico CrivellatoDomenico Ribatti
Mar 26, 2004·Journal of Autoimmunity·Julie CabarrocasRoland S Liblau
May 20, 2014·Journal of Neuroimmunology·Katarina RadojevićGordana Leposavić
Mar 21, 2002·Expert Opinion on Therapeutic Targets·Bela Bodey
Nov 4, 2008·Autonomic Neuroscience : Basic & Clinical·Gordana LeposavićDusko Kosec
Feb 12, 2008·Journal of Neuroimmunology·Ivan PilipovićGordana Leposavić
Sep 2, 2005·Journal of Neurochemistry·Susana Nieto-CerónFrancisco J Campoy
Nov 25, 2003·Microscopy Research and Technique·Renata Brelińska
Feb 15, 2018·Frontiers in Endocrinology·Gordana Momčilo Leposavić, Ivan M Pilipović

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