Abstract
Fasciola gigantica cathepsin-L cysteine proteinase and recombinant cathepsin L 1-D were assessed for their potential in the immuno-diagnosis of F. gigantica infection in buffaloes. A diagnostic ELISA, based on these two antigens, was developed to detect antibodies against F. gigantica in water buffaloes. Sensitivity of the ELISA was assessed using sera from buffaloes experimentally or naturally infected with F. gigantica from F. gigantica endemic areas and its specificity by probing the sera of the host from F. gigantica non-endemic area. Our earlier studies under experimental setting showed 100% sensitivity of cathepsin-L ELISA in the diagnosis of fasciolosis in buffaloes, with the earliest detection of infection at 4 weeks post-infection. However, under field situation of natural F. gigantica infection, this sensitivity declined to 97.1% but specificity of the test remained 100%. Cross-reactivity of the antigen was checked with Schistosoma indicum, S. spindale, Paramphistomum epiclitum, Gastrothylax spp., Gigantocotyle explanatum, hydatid and Strongyloides papilossus in the bubaline host, naturally infected with these helminths. F. gigantica cathepsin-L and the recombinant cathepsin L-1D does not cross-react with these helmin...Continue Reading
References
Sep 1, 1992·Veterinary Parasitology·D LevieuxA Venien
Jan 1, 1992·Parasitology Research·H YamasakiT Aoki
Jun 1, 1995·Veterinary Parasitology·B O FagbemiJ V Mbuh
Apr 1, 1995·Veterinary Parasitology·B O Fagbemi, E E Guobadia
Nov 1, 1993·Molecular and Biochemical Parasitology·A M SmithJ P Dalton
Mar 1, 1994·Experimental Parasitology·G L WijffelsF Bowen
Oct 15, 1996·Veterinary Parasitology·E E Guobadia, B O Fagbemi
Oct 1, 1996·Electrophoresis·B Finn, M Rowe
Feb 1, 1997·Veterinary Parasitology·E E Guobadia, B O Fagbemi
May 1, 1997·Veterinary Parasitology·B O FagbemiE E Guobadia
Jun 29, 1999·Veterinary Parasitology·S C YadavA K Verma
Jul 15, 1999·International Journal for Parasitology·J B CornelissenF J van Milligen
Sep 24, 1999·Transactions of the Royal Society of Tropical Medicine and Hygiene·M CórdovaJ R Espinoza
Oct 12, 2000·Veterinary Parasitology·R Sánchez-AndradeP Morrondo
Jan 4, 2001·Clinical and Diagnostic Laboratory Immunology·S CarnevaleE A Guarnera
May 5, 2001·International Journal for Parasitology·J B CornelissenW J Boersma
Jul 5, 2001·Parasitology International·R GramsV Viyanant
Nov 9, 2002·Veterinary Parasitology·A K DixitR L Sharma
Feb 26, 2004·Tropical Animal Health and Production·A K DixitR L Sharma
Jun 1, 2005·Veterinary Parasitology·J B MolloyB C Knight
Oct 14, 2005·Parasitology Research·S C YadavD Sriveny
Nov 23, 2005·Veterinary Parasitology·D SrivenyB P Singh
Oct 27, 2006·Epidemiology and Infection·M ParkinsonJ P Dalton
Citations
Mar 18, 2008·Parasitology Research·Niranjan KumarS C Gupta
Sep 11, 2008·Parasitology Research·Niranjan KumarS C Gupta
Nov 25, 2011·Parasitology Research·Gamal AllamThabet F Sakran
Jun 2, 2012·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·R L SharmaM B Thilagar
Jul 22, 2009·Journal of Helminthology·A JedeppaP S Banerjee
Mar 14, 2012·Journal of Helminthology·G AllamT F Sakran
Jan 27, 2012·Clinical and Vaccine Immunology : CVI·José F GaudierAna M Espino
Nov 21, 2009·The Journal of Parasitology·M AriasA Paz-Silva
Apr 12, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Eman H Abdel-RahmanEman E El Shanawany
Apr 17, 2014·Acta Tropica·Panat AnuracpreedaPrasert Sobhon
Dec 28, 2010·Parasitology International·Weiyu ZhangEmmanuelle Moreau
Aug 13, 2008·Molecular and Biochemical Parasitology·Marcel Marín-VillaYara M Traub-Csekö
Sep 28, 2007·Veterinary Parasitology·R El RidiH Motawi
Jun 6, 2007·Biopolymers·Jan JezekVassilios Tsikaris
Dec 15, 2010·Veterinary Parasitology·Abhishek GuptaR L Sharma
Nov 8, 2011·Veterinary Parasitology·Anju VargheseJustin D Kollannur
Jan 6, 2016·PloS One·Panat AnuracpreedaPrasert Sobhon
Aug 16, 2013·Clinical and Vaccine Immunology : CVI·Abdelfattah M AttallahHisham Ismail
Dec 20, 2013·Journal of Clinical Microbiology·Kimberly Cabán-HernándezAna M Espino
Dec 1, 2017·Veterinary World·Niranjan KumarJ B Solanki
Feb 23, 2020·Acta Parasitologica·Andleeb AftabO K Raina
Oct 4, 2017·PloS One·M A Hannan KhanS M A Abidi
Jan 15, 2021·Experimental Parasitology·H LalrinkimaM C Lallianchhunga
Apr 22, 2018·Experimental Parasitology·Behnam MeshgiZahra Jahani