PMID: 3610418Jan 1, 1987Paper

Immuno-enhancing activity of the amino-terminal domain of human prealbumin: isolation, characterization and synthesis

International Journal of Immunopharmacology
P M BurtonA White

Abstract

A decapeptide isolated from highly purified preparations of human prealbumin was able to restore azathioprine (Az) sensitivity, a property of a sub-class of T-lymphocytes, to the spleen rosette-forming cells (RFC) of adult thymectomized (ATx) mice in vitro. The peptide was sequenced by the Edman method and shown to correspond to the ten amino-terminal residues of prealbumin, Gly-Pro-Thr-Gly-Thr-Gly-Glu-Ser-Lys-Cys. Synthesis of this peptide by solid phase methodology confirmed its activity both in vitro and in vivo. Synthesis of a number of structural analogues indicated that the amino-terminal deca, undeca and dodecapeptides of prealbumin as well as some of their derivatives were also able to restore Az sensitivity to RFC in vitro and in vivo. The Cys10 residue and the Glu7 residues both contributed significantly to potency in vitro. Removal of up to three amino acids from the N-terminus of the decapeptide led to a progressive loss of activity. The data indicates that the ability of human prealbumin to restore the Az sensitivity to the RFC of adult Tx mice is intrinsic to the protein and resides in the amino-terminal domain of the molecule.

References

Apr 22, 1976·Nature·A AstaldiV P Eijswoogel
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A L GoldsteinJ Meienhofer
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P M BurtonA White
Jan 1, 1979·Annals of the New York Academy of Sciences·A White, P M Burton
Jan 1, 1985·International Journal of Immunopharmacology·P M BurtonA White
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J F BachA White
May 26, 1971·Nature: New Biology·J F BachA J Davies
Sep 1, 1981·The Journal of Experimental Medicine·M PrasB Frangione
Feb 1, 1980·European Journal of Immunology·M DardenneJ F Bach

Citations

Jan 1, 1989·Virchows Archiv. A, Pathological Anatomy and Histopathology·B Jacobsson
May 1, 1992·Brain Research. Brain Research Reviews·C NilssonCh Owman
Jan 30, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Joana Almeida Palha
Jan 16, 1989·Biochemical and Biophysical Research Communications·T PetterssonH Jörnvall
Jul 1, 1990·Medicinal Research Reviews·V St Georgiev
Jan 1, 1991·Medicinal Research Reviews·V St Georgiev
Mar 29, 2014·Perceptual and Motor Skills·Miguelina GuiraoAmalia Calviño

Related Concepts

Metazoa
Azathioprine sodium
High Pressure Liquid Chromatography Procedure
Mice, Inbred C57BL
Peptide Fragments
Prealbumin
Immunocytoadherence
Spleen
Mouse, Swiss
In Vitro [Publication Type]

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