Immuno-imaging of ICAM-1 in tumours by SPECT.

Nuclear Medicine and Biology
Michael MosleyBart Cornelissen

Abstract

Molecular imaging of cancer cells' reaction to radiation damage can provide a non-invasive measure of tumour response to treatment. The cell surface glycoprotein ICAM-1 (CD54) was identified as a potential radiation response marker. SPECT imaging using an 111In-radiolabelled anti-ICAM-1 antibody was explored. PSN-1 cells were irradiated (10 Gy), and protein expression changes were investigated using an antibody array on cell lysates 24 h later. Results were confirmed by western blot, flow cytometry and immunofluorescence. We confirmed the affinity of an 111In-labelled anti-ICAM-1 antibody in vitro, and in vivo, in PSN-1-xenograft bearing mice. The xenografts were irradiated (0 or 10 Gy), and [111In]In-anti-ICAM-1 SPECT/CT images were acquired 24, 48 and 72 h after intravenous administration. ICAM-1 was identified as a potential marker of radiation treatment using an antibody array in PSN-1 cell lysates following irradiation, showing a significant increase in ICAM-1 signal compared to non-irradiated cells. Western blot and immunohistochemistry confirmed this upregulation, with an up to 20-fold increase in ICAM-1 signal. Radiolabelled anti-ICAM-1 bound to ICAM-1 expressing cells with good affinity (Kd = 24.0 ± 4.0 nM). [111In]In-...Continue Reading

Citations

Apr 1, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yang ZhaoZhaofei Liu
Feb 5, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Miao LiWeibo Cai

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Methods Mentioned

BETA
imaging techniques
antibody array
xenograft
Profiler
xenografts
fluorescence microscopy
X-ray
flow
saturation binding

Software Mentioned

Matlab
FlowJo
GraphPad
PMod
ImageJ
SROSEM
GraphPad Prism

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