Immuno-modified superparamagnetic nanoparticles via host-guest interactions for high-purity capture and mild release of exosomes

Nanoscale
Shuang CaiYao Wu

Abstract

The study of exosomes has great significance for cancer diagnosis and prognosis, in which damage-free isolation of exosomes is a critically important step due to their extremely low concentration in body fluids. Herein, immunoaffinitive superparamagnetic nanoparticles (IS-NPs) were successfully constructed by combining antibodies with superparamagnetic nanoparticles through host-guest interactions between β-cyclodextrin (β-CD) and 4-aminoazobenzene (AAB). Exosomes in cell-culture supernatants or body fluids can be efficiently captured by IS-NPs and then mildly eluted by competitive host molecules, α-CD. Approximately 80% of the exosomes were captured by the IS-NPs from the model samples containing varied concentrations of exosomes (1011-107 exosomes per mL). After elution, the release efficiency of the captured exosomes was as high as 86.5%, as calculated from the extracted RNA content. A comparison between the exosomes isolated by our IS-NPs, conventional ultracentrifugation (UC), polyethylene glycol (PEG)-based precipitation, and a commercial kit was conducted to examine the performance of the IS-NP exosome isolation. The particle-to-protein ratio of IS-NP captured exosomes (8.8 ± 1.3 × 109) was eight-fold higher than that of...Continue Reading

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Jul 4, 2019·Analytical and Bioanalytical Chemistry·Rui HouYukui Zhang
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Methods Mentioned

BETA
scanning
transmission electron microscopy
dynamic light scattering
electrophoresis
fluorescence spectroscopy
electron microscopy
confocal microscopy

Software Mentioned

Adobe Illustrator
Proteome Discoverer
MASCOT
Agilent 2100 Expert
NTA

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