DOI: 10.1101/471821Nov 19, 2018Paper

Immuno-proteomic interrogation of dengue infection reveals novel HLA haplotype-specific MHC-I antigens

BioRxiv : the Preprint Server for Biology
Kavya SwaminathanJoshua E. Elias

Abstract

Broadly effective vaccines against dengue virus (DENV) infection have remained elusive, despite rising infection rates in the developing world. Infection-specific peptide ligands presented on Major Histocompatibility Complexes (MHC) open new avenues for developing T-cell-based interventions. Past efforts towards mapping viral MHC epitopes were based on computational predictions that only partially reflected actual antigen presentation. To empirically identify DENV-specific MHC ligands, we developed an immuno-proteomics approach for interrogating DENV- and self-derived MHC ligands from infected B-lymphocytes. Here, we report four fundamental findings: First, over 700 infection-specific MHC-ligands reflected host cellular responses to DENV that were not apparent from the proteome. Second, we report 121 viral MHC-I ligands (108 novel) which clustered into discrete hotspots across the DENV polyprotein, some of which spanned DENV polyprotein components, described here as MHC ligands for the first time. Third, we found DENV ligands which were distinctly presented by MHC alleles previously associated with either high or low anti-DENV response. Fourth, we demonstrate that while our in vitro assay only overlapped with a small fraction o...Continue Reading

Related Concepts

Epitopes
Antigens
B-Lymphocytes
Cytology
Dengue Fever
Dengue Virus
HLA Antigens
Ligands
T-Lymphocyte
Vaccines

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