Immunoadjuvant chaperone, GRP170, induces 'danger signals' upon interaction with dendritic cells

Immunology and Cell Biology
Masoud H ManjiliJ R Subjeck

Abstract

When chaperoning tumour antigens, glucose-regulated protein 170 (GRP170) is capable of inducing effective antitumour immune responses. In the present study, we determined whether such immunoadjuvant properties of GRP170 also involve the ability to induce 'danger signals' through interaction with APC. We prepared recombinant GRP170 in the baculovirus expression system with low endotoxin concentration at which LPS did not have any effect on dendritic cells (DC). We showed that GRP170 binds DC in a receptor-mediated fashion and induces DC to upregulate the expression of MHC class II, CD86 and CD40 molecules, and to secrete pro-inflammatory cytokines. GRP170 also induced expression of CD40 molecules in a B16F10 cell line, whereas LPS failed to do so. These findings show that GRP170 acts as a danger signal through its interaction with DC, regardless of its endotoxin component.

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Citations

Jan 15, 2009·Cancer Immunology, Immunotherapy : CII·Ping GaoXiang-Yang Wang
Sep 5, 2008·Expert Review of Vaccines·Ayesha MurshidStuart K Calderwood
Mar 15, 2016·Frontiers in Immunology·Daming ZuoXiang-Yang Wang
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May 21, 2016·Frontiers in Immunology·Stuart K CalderwoodAyesha Murshid
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Sep 16, 2017·International Journal of Molecular Sciences·Suman Chatterjee, Timothy F Burns
Sep 1, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Stefano ToldoAntonio Abbate

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