Immunoaffinity chromatography: an introduction to applications and recent developments.

Bioanalysis
Annette C Moser, David S Hage

Abstract

Immunoaffinity chromatography (IAC) combines the use of LC with the specific binding of antibodies or related agents. The resulting method can be used in assays for a particular target or for purification and concentration of analytes prior to further examination by another technique. This review discusses the history and principles of IAC and the various formats that can be used with this method. An overview is given of the general properties of antibodies and of antibody-production methods. The supports and immobilization methods used with antibodies in IAC and the selection of application and elution conditions for IAC are also discussed. Several applications of IAC are considered, including its use in purification, immunodepletion, direct sample analysis, chromatographic immunoassays and combined analysis methods. Recent developments include the use of IAC with CE or MS, ultrafast immunoextraction methods and the use of immunoaffinity columns in microanalytical systems.

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Citations

Feb 22, 2012·Biotechnology Letters·Eiry KobatakeMasayasu Mie
Mar 25, 2014·Biotechnology Letters·Dongli Guan, Zhilei Chen
Feb 28, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiwei ZhengDavid S Hage
Sep 30, 2014·Journal of Chromatography. a·Erika L PfaunmillerDavid S Hage
Feb 13, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chaochao WuWei-Jun Qian
Mar 6, 2012·Journal of Pharmaceutical and Biomedical Analysis·E CalleriG Massolini
Feb 7, 2012·Journal of Pharmaceutical and Biomedical Analysis·David S HageXiwei Zheng
Dec 26, 2015·Analytica Chimica Acta·Sara PorfírioParastoo Azadi
Jan 22, 2015·MAbs·Pauline BonvinNicolas Fischer
Dec 15, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Norberto A Guzman, Daniel E Guzman
Oct 18, 2013·Electrophoresis·Annette C MoserDavid S Hage
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Methods Mentioned

BETA
reversed-phase chromatography
immunodepletion
sandwich immunoassay
Assay
Fluorescence

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