Immunoaffinity column clean-up for the high-performance liquid chromatographic determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in urine

Journal of Chromatography. B, Biomedical Applications
A KussakK Andersson

Abstract

A method for the determination of aflatoxins B1, B2, G1, G2, M1 and Q1 in human urine has been developed. The 10-ml urine samples were automatically cleaned up on immunoaffinity columns and analysed by high-performance liquid chromatography (HPLC), including post-column derivatization with bromine and fluorescence detection. Average aflatoxin recoveries were: B1 103%, B2 106%, G1 98% and G2 96% in the range 6.8-73 pg/ml of urine and M1 103% and Q1 100% in the range 18-97 pg/ml of urine. The relative standard deviations were all between 1% and 21%. The determination limits of aflatoxins in urine were 6.8 pg/ml for B1, B2, G1 and G2 and 18 pg/ml for M1 and Q1.

References

Sep 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·C I WeiD P Hsieh
Sep 23, 1994·Journal of Chromatography. B, Biomedical Applications·W T Kok

❮ Previous
Next ❯

Citations

Jun 2, 2009·Analytical and Bioanalytical Chemistry·Gordon S Shephard
Mar 17, 1999·Toxicon : Official Journal of the International Society on Toxinology·K KawatsuY Hamano
Nov 29, 2002·Food Additives and Contaminants·A GovarisN A Botsoglou
Oct 25, 2002·Food Additives and Contaminants·V RoussiN A Botsoglou
Mar 1, 2012·Food Additives & Contaminants. Part B, Surveillance·Samir AbbèsRidha Oueslati
Jan 1, 1995·Rapid Communications in Mass Spectrometry : RCM·A KussakJ Langridge
Nov 6, 2013·Critical Reviews in Food Science and Nutrition·Jinyi YangDharini Sivakumar
Dec 16, 2005·Photochemistry and Photobiology·WenLi ChenWeiGui Chen
Apr 1, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·W L ShelverJ K Huwe
Aug 11, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·P SimonT Nicot

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.