Immunoaffinity isolation and partial characterization of the Coccidioides immitis antigen detected by the tube precipitin and immunodiffusion-tube precipitin tests.

Journal of Clinical Microbiology
B L Zimmer, D Pappagianis

Abstract

The antigen participating in the tube precipitin (TP) serologic test for coccidioidomycosis was isolated from mycelial-phase antigen (coccidioidin) by immunoaffinity and characterized by various analytical procedures. This was accomplished by first preparing the antigen-antibody precipitate by using antigen and human serum positive for TP (immunoglobulin M) antibody and then liberating the antigen by digestion with pronase. This protease destroyed the antibody and left the antigen intact as indicated by immunodiffusion-TP. The coccidioidal antigen was isolated from the proteolytic digest by using size exclusion chromatography. DEAE chromatography of this antigen yielded two fractions with immunodiffusion-TP reactivity which had average molecular sizes of 225 and 140 kilodaltons, respectively. The presence of carbohydrate and amino acids indicated that the antigen(s) is a glycopeptide. Compositional analysis showed that one fraction contained 3-O-methylmannose, mannose, and glucose in a ratio of 8:1.2:1, whereas the second fraction contained 3-O-methylmannose, mannose, glucose, and galactose in a ratio of 1:1:1:1. The amino acids glycine, alanine, serine, threonine, aspartic acid plus asparagine, and glutamic acid plus glutamine...Continue Reading

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Citations

Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·L De RepentignyR C Matthews
Mar 24, 2005·Infection and Immunity·Neil M AmpelDemosthenes Pappagianis
Mar 30, 2001·Fungal Genetics and Biology : FG & B·D Pappagianis
Jul 1, 1991·Infection and Immunity·K O DuggerJ H Law
Jan 1, 1990·Infection and Immunity·D Kruse, G T Cole
Jul 1, 1990·Clinical Microbiology Reviews·D Pappagianis, B L Zimmer

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